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PSAP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS344078

Intended Use: The PSAP [IHC655] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test… More details

$205 / 0.1 mL (Concentrate) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PSAP, Monoclonal Antibody
Full Product Name
PSAP
Product Gene Name
anti-PSAP antibody
Clonality
Monoclonal
Host
Mouse
Species Reactivity
Human
Form/Format
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
Positive and negative controls should be simultaneously run with unknown specimens.
Molecular Weight
58,356 Da
3D Structure
ModBase 3D Structure for P07602
Positive Control
Prostate, Prostate Adenocarcinoma
Principles and Procedures
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
Reconstitution
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
Specimen Collection and Preparation for Analysis
Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).
Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
Application Notes for anti-PSAP antibody
The recommended working dilution range is 1:100 - 1:200.

Immunohistochemistry (IHC) of anti-PSAP antibody

PSAP [IHC655] on Prostate
Mouse Anti-PSAP Monoclonal Antibody – Cat# MBS344078

Product References and Citations for anti-PSAP antibody

1. Ansari MA, et al. Am J Clin Path. 1981; 76:94-8.
2. Nadji M, et al. Ann N Y Acad Sci. 1982; 390:133-41.
3. Kimura N, et al. Virchows Arch A Pathol Anat Histopathol. 1986; 410:247-51.
4. Kidwai N, et al. Breast Cancer Res. 2004; 6:R18-23.
5. Kuroda N, et al. Pathol Int. 1999; 49:457-61.
6. Elgamal AA, et al. Urology. 1994; 44:84-90.
7. Gatalica Z, et al. Appl Immunohistochem Mol Morphol. 2000; 8:158-61.
8. Genega EM, et al. Mod Pathol. 2000; 13:1186-91.
9. Green LK, et al. Hum Pathol. 1991; 22:242-6.
10. Epstein JI. Urol Clin North Am. 1993; 20:757-70.
11. van Krieken JH. Am J Surg Pathol. 1993; 17:410-4.

Other Notes

Small volumes of anti-PSAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PSAP purchase

MBS344078 is an antibody targeting PSAP. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PSAP. It may not necessarily be applicable to this product.

NCBI GI #
48145609
NCBI GeneID
5660
NCBI Accession #
CAG33027.1
UniProt Primary Accession #
P07602
UniProt Secondary Accession #
P07292; P15793; P78538; P78541; P78546; P78547; P78558; Q53Y86; Q6IBQ6; Q92739; Q92740
UniProt Related Accession #
P07602
NCBI Official Full Name
PSAP
NCBI Official Synonym Full Names
prosaposin
NCBI Official Symbol
PSAP
NCBI Official Synonym Symbols
GLBA; SAP1
UniProt Protein Name
Prosaposin
UniProt Synonym Protein Names
Proactivator polypeptide
Protein Family
Prosaposin
UniProt Gene Name
PSAP
UniProt Synonym Gene Names
GLBA; SAP1; CSAct; SAP-1; SAP-2
NCBI Protein Information
prosaposin

NCBI Summary for PSAP

This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
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UniProt Comments for PSAP

Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.
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Research Articles on PSAP

1. Both PSAP reduction and overexpression lead to significantly elevated extracellular progranulin (PGRN) levels. Intriguingly, PSAP knockdown increases PGRN monomers, whereas PSAP overexpression increases PGRN oligomers, partly through a protein-protein interaction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PSAP antibody

 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram

Diseases associated with anti-PSAP antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >123 publications with PSAP and Nervous System Diseases
 Brain Diseases Antibodies  >103 publications with PSAP and Brain Diseases
 Neoplasms Antibodies  >90 publications with PSAP and Neoplasms
 Metachromatic Leukodystrophy due to Saposin B Deficiency Antibodies  >26 publications with PSAP and Metachromatic Leukodystrophy due to Saposin B Deficiency
 Skin Diseases Antibodies  >23 publications with PSAP and Skin Diseases
 Liver Diseases Antibodies  >19 publications with PSAP and Liver Diseases
 Cardiovascular Diseases Antibodies  >17 publications with PSAP and Cardiovascular Diseases
 Hyperplasia Antibodies  >13 publications with PSAP and Hyperplasia
 Breast Neoplasms Antibodies  >12 publications with PSAP and Breast Neoplasms
 Nerve Degeneration Antibodies  >10 publications with PSAP and Nerve Degeneration

Organs/Tissues associated with anti-PSAP antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >96 publications with PSAP and Brain
 Prostate Antibodies  >79 publications with PSAP and Prostate
 Blood Antibodies  >63 publications with PSAP and Blood
 Liver Antibodies  >48 publications with PSAP and Liver
 Nerve Antibodies  >44 publications with PSAP and Nerve
 Testis Antibodies  >42 publications with PSAP and Testis
 Kidney Antibodies  >37 publications with PSAP and Kidney
 Skin Antibodies  >32 publications with PSAP and Skin
 Spleen Antibodies  >25 publications with PSAP and Spleen
 Muscle Antibodies  >18 publications with PSAP and Muscle
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