• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Arginase-1 (ARG1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS346002

Arginse-1 is a key enzyme in the urea cycle, catalyzing the final step of hydrolysis of arginine to ornithine and urea. Anti-Arginase-1 antibodies are highly specific for hepatocytes and can be used as a marker for benign and malignant hepatic tumors. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
Arginase-1 (Arg1), Monoclonal Antibody
Full Product Name
Arginase-1 Antibody
Product Gene Name
anti-Arg1 antibody
Clonality
Monoclonal
Isotype
IgG
Clone Number
IHC400
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
Buffer
Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
IHC Positive Control
Liver
IHC Suggested Protocol
1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)
Applications Tested/Suitable for anti-Arg1 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-Arg1 antibody
Dilution Range: 1:100 - 1:200

Immunohistochemistry (IHC) of anti-Arg1 antibody

IHC (paraffin) of human colon using Arginase-1 Antibody
Mouse Anti-ARG1 Monoclonal Antibody – Cat# MBS346002

Other Notes

Small volumes of anti-Arg1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Arg1 purchase

MBS346002 is an antibody targeting Arg1. Also known as Arginase-1 Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Arg1. It may not necessarily be applicable to this product.

NCBI GI #
528078175
NCBI GeneID
101825450
NCBI Accession #
NP_001268574.1
GenBank Nucleotide #
NM_001281645.1
NCBI Official Full Name
arginase-1
NCBI Official Symbol
Arg1
NCBI Official Synonym Symbols
arginase-1
Protein Family
Arginase
NCBI Protein Information
arginase-1

Research Articles on Arg1

1. Data suggest Fgfr1 (fibroblast growth factor receptor 1) and Igf1r (insulin-like growth factor 1 receptor) signaling via Stat6 (signal transducer/activator of transcription 6) is involved in Arg1 induction in macrophages/Th2 cells in leishmaniasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000