Mouse Anti-BSEP Monoclonal Antibody – Cat# MBS346003

IHC (paraffin) of human liver using BSEP Antibody

BSEP Antibody – Mouse Monoclonal

Name: BSEP Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS346003
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS346003

Bile Salt Export Pump (BSEP, also known as ABCB11) belongs to the ATP-binding cassette (ABC) transporters, which takes part in transporting bile acid, taurocholate, and other cholate conjugates across the hepatocyte canalicular membrane into the canaliculus.… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

BSEP, Monoclonal Antibody

Full Product Name

BSEP Antibody

Product Gene Name

anti-BSEP antibody

Clonality

Monoclonal

Isotype

IgG

Clone Number

IHC518

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid

Preparation and Storage

Store at 2-8 degree C. Do not freeze.

Molecular Weight

146,407 Da

3D Structure

ModBase 3D Structure for O95342

Buffer

Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3

IHC Positive Control

Liver

IHC Suggested Protocol

1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)

Applications Tested/Suitable for anti-BSEP antibody

Immunohistochemistry (IHC) Paraffin

Application Notes for anti-BSEP antibody

Dilution Range: 1:100 - 1:200

Other Notes

Small volumes of anti-BSEP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BSEP purchase

MBS346003 is an antibody targeting BSEP. Also known as BSEP Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BSEP. It may not necessarily be applicable to this product.

NCBI GI #

21536378

NCBI GeneID

8647

NCBI Accession #

NP_003733.2

UniProt Primary Accession #

O95342

UniProt Secondary Accession #

Q53TL2; Q9UNB2

UniProt Related Accession #

O95342

GenBank Nucleotide #

NM_003742.4

NCBI Official Full Name

bile salt export pump

NCBI Official Synonym Full Names

ATP binding cassette subfamily B member 11

NCBI Official Symbol

ABCB11

NCBI Official Synonym Symbols

BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2

UniProt Protein Name

Bile salt export pump

UniProt Synonym Protein Names

ATP-binding cassette sub-family B member 11

UniProt Gene Name

ABCB11

UniProt Synonym Gene Names

BSEP

NCBI Protein Information

bile salt export pump

NCBI Summary for BSEP

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008]

UniProt Comments for BSEP

Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.

Research Articles on BSEP

1. This is the first report on a diagnostic test for simultaneous genotyping of IFNL3, ABCB11, and RNF7 in Chronic hepatitis C (CHC) patients. Reliable and inexpensive, the assay should provide useful information for the clinical management of CHC, like identification of patients at risk of rapid disease progression or with high chances of response to classic therapy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BSEP antibody

Reference Product	PubMed Publications
ATP8B1 antibody	>83 publications with BSEP and ATP8B1
CYP7A1 antibody	>48 publications with BSEP and CYP7A1
SLC10A1 antibody	>30 publications with BSEP and SLC10A1
SLCO1B1 antibody	>16 publications with BSEP and SLCO1B1
CYP8B1 antibody	>13 publications with BSEP and CYP8B1
SLCO1B3 antibody	>11 publications with BSEP and SLCO1B3
NR1H4 antibody	>9 publications with BSEP and NR1H4
NR0B2 antibody	>3 publications with BSEP and NR0B2

Pathways associated with anti-BSEP antibody

Products by Pathway	Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
Bile Acid And Bile Salt Metabolism Pathway antibodies	Bile Acid And Bile Salt Metabolism Pathway Diagram
Bile Secretion Pathway antibodies	Bile Secretion Pathway Diagram
Bile Secretion Pathway antibodies	Bile Secretion Pathway Diagram
Drug Induction Of Bile Acid Pathway antibodies	Drug Induction Of Bile Acid Pathway Diagram
Endocrine Resistance Pathway antibodies	Endocrine Resistance Pathway Diagram
Endocrine Resistance Pathway antibodies	Endocrine Resistance Pathway Diagram
Farnesoid X Receptor Pathway antibodies	Farnesoid X Receptor Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram

Diseases associated with anti-BSEP antibody

Disease Name	Pubmed Publications
Cholestasis Antibodies	>388 publications with BSEP and Cholestasis
Liver Diseases Antibodies	>329 publications with BSEP and Liver Diseases
Cholestasis, Intrahepatic Antibodies	>265 publications with BSEP and Cholestasis, Intrahepatic
Drug-Related Side Effects and Adverse Reactions Antibodies	>50 publications with BSEP and Drug-Related Side Effects and Adverse Reactions
Chemical and Drug Induced Liver Injury Antibodies	>49 publications with BSEP and Chemical and Drug Induced Liver Injury
Pruritus Antibodies	>48 publications with BSEP and Pruritus
Liver Cirrhosis Antibodies	>44 publications with BSEP and Liver Cirrhosis
Inflammation Antibodies	>41 publications with BSEP and Inflammation
Hepatitis Antibodies	>37 publications with BSEP and Hepatitis
Jaundice Antibodies	>36 publications with BSEP and Jaundice