Mouse Anti-KRT14 Monoclonal Antibody – Cat# MBS346009

IHC (paraffin) of human colon using Cytokeratin 14 Antibody

Cytokeratin 14 (KRT14) Antibody – Mouse Monoclonal

Name: KRT14 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS346009
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS346009

CK14 (Cytokeratin 14) is part of the type I keratin family proteins, and can be found in squamous epithelial basal cells, myoepithelium, some glandular epithelia, and mesothelial cells. Anti-Cytokeratin 14 antibodies can be useful in identifying squamous cell… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Cytokeratin 14 (KRT14), Monoclonal Antibody

Full Product Name

Cytokeratin 14 Antibody

Product Gene Name

anti-KRT14 antibody

Clonality

Monoclonal

Isotype

IgG

Clone Number

IHC555

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid

Preparation and Storage

Store at 2-8 degree C. Do not freeze.

Molecular Weight

51,561 Da

3D Structure

ModBase 3D Structure for P02533

Buffer

Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3

IHC Positive Control

Squamous Cell Carcinoma

IHC Suggested Protocol

1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)

Applications Tested/Suitable for anti-KRT14 antibody

Immunohistochemistry (IHC) Paraffin

Application Notes for anti-KRT14 antibody

Dilution Range: 1:100 - 1:200

Other Notes

Small volumes of anti-KRT14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT14 purchase

MBS346009 is an antibody targeting KRT14. Also known as Cytokeratin 14 Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT14. It may not necessarily be applicable to this product.

NCBI GI #

355568680

NCBI Accession #

EHH24961.1

UniProt Primary Accession #

P02533

UniProt Secondary Accession #

Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4

NCBI Official Full Name

Cytokeratin-14

UniProt Protein Name

Keratin, type I cytoskeletal 14

UniProt Synonym Protein Names

Cytokeratin-14; CK-14; Keratin-14; K14

Protein Family

Keratin

UniProt Gene Name

KRT14

UniProt Synonym Gene Names

CK-14; K14

UniProt Comments for KRT14

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT14 antibody

Reference Product	PubMed Publications
KRT5 antibody	>120 publications with KRT14 and KRT5
KRT6A antibody	>5 publications with KRT14 and KRT6A
DST antibody	>4 publications with KRT14 and DST
KRT6B antibody	>4 publications with KRT14 and KRT6B
FLG antibody	>3 publications with KRT14 and FLG
IVL antibody	>1 publications with KRT14 and IVL
KRT3 antibody	>1 publications with KRT14 and KRT3
CREBBP antibody	>1 publications with KRT14 and CREBBP

Diseases associated with anti-KRT14 antibody

Disease Name	Pubmed Publications
Congenital Abnormalities Antibodies	>116 publications with KRT14 and Congenital Abnormalities
Epidermolysis Bullosa Simplex Antibodies	>111 publications with KRT14 and Epidermolysis Bullosa Simplex
Carcinoma, Squamous Cell Antibodies	>39 publications with KRT14 and Carcinoma, Squamous Cell
Breast Neoplasms Antibodies	>22 publications with KRT14 and Breast Neoplasms
Hyperplasia Antibodies	>21 publications with KRT14 and Hyperplasia
Inflammation Antibodies	>18 publications with KRT14 and Inflammation
Necrosis Antibodies	>14 publications with KRT14 and Necrosis
Cell Transformation, Neoplastic Antibodies	>13 publications with KRT14 and Cell Transformation, Neoplastic
Pigmentation Disorders Antibodies	>11 publications with KRT14 and Pigmentation Disorders
Fibrosis Antibodies	>11 publications with KRT14 and Fibrosis