Mouse Anti-KRT19 Monoclonal Antibody – Cat# MBS346011

IHC (paraffin) of human colon using Cytokeratin 19 Antibody

Cytokeratin 19 (KRT19) Antibody – Mouse Monoclonal

Name: KRT19 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS346011
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS346011

CK19 (Cytokeratin 19) is part of the type I keratin family proteins, its filaments can be found in epithelial tissues and provides mechanical support for cells. Anti-Cytokeratin 19 antibodies can be used to stain carcinomas of the colon, stomach, pancreas,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Cytokeratin 19 (KRT19), Monoclonal Antibody

Full Product Name

Cytokeratin 19 Antibody

Product Gene Name

anti-KRT19 antibody

Clonality

Monoclonal

Isotype

IgG

Clone Number

IHC019

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid

Preparation and Storage

Store at 2-8 degree C. Do not freeze.

Molecular Weight

44,106 Da

Buffer

Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3

IHC Positive Control

Bladder, Colon Carcinoma, Colon, Thyroid Carcinoma

IHC Suggested Protocol

1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)

Applications Tested/Suitable for anti-KRT19 antibody

Immunohistochemistry (IHC) Paraffin

Application Notes for anti-KRT19 antibody

Dilution Range: 1:100 - 1:200

Other Notes

Small volumes of anti-KRT19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT19 purchase

MBS346011 is an antibody targeting KRT19. Also known as Cytokeratin 19 Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT19. It may not necessarily be applicable to this product.

NCBI GI #

13537138

NCBI GeneID

3880

NCBI Accession #

BAB40770.1

UniProt Secondary Accession #

Q5XG83; Q6NW33; Q7L5M9; Q96A53; Q96FV1; Q9BYF9; Q9P1Y4; B2R874

UniProt Related Accession #

P08727

NCBI Official Full Name

cytokeratin 19, partial

NCBI Official Synonym Full Names

keratin 19

NCBI Official Symbol

KRT19

NCBI Official Synonym Symbols

K19; CK19; K1CS

UniProt Protein Name

Keratin, type I cytoskeletal 19

UniProt Synonym Protein Names

Cytokeratin-19; CK-19; Keratin-19; K19

Protein Family

Keratin

UniProt Gene Name

KRT19

UniProt Synonym Gene Names

CK-19; K19

NCBI Protein Information

keratin, type I cytoskeletal 19

NCBI Summary for KRT19

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

UniProt Comments for KRT19

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Research Articles on KRT19

1. KRT19 intracellularly binds to HER2, playing a critical role in HER2 activation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT19 antibody

Reference Product	PubMed Publications
EPCAM antibody	>24 publications with KRT19 and EPCAM
KRT8 antibody	>17 publications with KRT19 and KRT8
MUC1 antibody	>13 publications with KRT19 and MUC1
AFP antibody	>8 publications with KRT19 and AFP
CEACAM5 antibody	>7 publications with KRT19 and CEACAM5
TP53 antibody	>5 publications with KRT19 and TP53
MAPK14 antibody	>1 publications with KRT19 and MAPK14

Pathways associated with anti-KRT19 antibody

Products by Pathway	Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Keratinization Pathway antibodies	Keratinization Pathway Diagram
Signaling Mediated By P38-alpha And P38-beta Pathway antibodies	Signaling Mediated By P38-alpha And P38-beta Pathway Diagram

Diseases associated with anti-KRT19 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>27 publications with KRT19 and Adenocarcinoma
Breast Neoplasms Antibodies	>22 publications with KRT19 and Breast Neoplasms
Liver Diseases Antibodies	>21 publications with KRT19 and Liver Diseases
Liver Neoplasms Antibodies	>15 publications with KRT19 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>15 publications with KRT19 and Carcinoma, Hepatocellular
Lung Neoplasms Antibodies	>8 publications with KRT19 and Lung Neoplasms
Neoplasm Invasiveness Antibodies	>7 publications with KRT19 and Neoplasm Invasiveness
Fibrosis Antibodies	>7 publications with KRT19 and Fibrosis
Inflammation Antibodies	>6 publications with KRT19 and Inflammation
Neoplasms, Experimental Antibodies	>6 publications with KRT19 and Neoplasms, Experimental