Mouse Anti-TFE3 Monoclonal Antibody – Cat# MBS346035

IHC (paraffin) of human kidney using TFE3 Antibody

TFE3 Antibody – Mouse Monoclonal

Name: TFE3 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS346035
Price: 490 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS346035

TFE3 (Transcription Factor E3) is a transcription factor that binds to the MUE3-type E-box sequences involved in TGF-beta signalling. Anti-TFE3 antibody staining is the most sensitive and specific indicator of Xp11 translocation renal cell carcinomas. Since… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TFE3, Monoclonal Antibody

Full Product Name

TFE3 Antibody

Product Gene Name

anti-TFE3 antibody

Clonality

Monoclonal

Isotype

IgG

Clone Number

IHC672

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid

Preparation and Storage

Store at 2-8 degree C. Do not freeze.

Molecular Weight

11,363 Da

3D Structure

ModBase 3D Structure for P19532

Buffer

Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3

IHC Positive Control

Testes, Melanoma, Xp11.2 Translocation Renal Cell Carcinoma

IHC Suggested Protocol

1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)

Applications Tested/Suitable for anti-TFE3 antibody

Immunohistochemistry (IHC) Paraffin

Application Notes for anti-TFE3 antibody

Dilution Range: 1:100 - 1:200

Other Notes

Small volumes of anti-TFE3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFE3 purchase

MBS346035 is an antibody targeting TFE3. Also known as TFE3 Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TFE3. It may not necessarily be applicable to this product.

NCBI GI #

531034764

NCBI GeneID

7030

NCBI Accession #

NP_001269071.1

UniProt Primary Accession #

P19532

UniProt Secondary Accession #

Q5JU74; Q92757; Q92758; Q99964; A8MZL6

UniProt Related Accession #

P19532

GenBank Nucleotide #

NM_001282142.1

NCBI Official Full Name

transcription factor E3 isoform 2

NCBI Official Synonym Full Names

transcription factor binding to IGHM enhancer 3

NCBI Official Symbol

TFE3

NCBI Official Synonym Symbols

TFEA; RCCP2; RCCX1; bHLHe33

UniProt Protein Name

Transcription factor E3

UniProt Synonym Protein Names

Class E basic helix-loop-helix protein 33; bHLHe33

Protein Family

Transcription factor

UniProt Gene Name

TFE3

UniProt Synonym Gene Names

BHLHE33; bHLHe33

NCBI Protein Information

transcription factor E3

NCBI Summary for TFE3

This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

UniProt Comments for TFE3

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.

Research Articles on TFE3

1. Both SOX11 and TFE3 were overexpressed in solid-pseudopapillary neoplasms (SPNs) and may be involved in the pathogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TFE3 antibody

Reference Product	PubMed Publications
TFEB antibody	>122 publications with TFE3 and TFEB
MITF antibody	>104 publications with TFE3 and MITF
PRCC antibody	>57 publications with TFE3 and PRCC
ASPSCR1 antibody	>53 publications with TFE3 and ASPSCR1
TFEC antibody	>27 publications with TFE3 and TFEC
SMAD3 antibody	>7 publications with TFE3 and SMAD3
SMAD4 antibody	>5 publications with TFE3 and SMAD4
E2F3 antibody	>3 publications with TFE3 and E2F3

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TGF-beta Receptor Signaling Pathway antibodies	TGF-beta Receptor Signaling Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
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