Botox-E Antibody – Monoclonal

Immunofluorescent: This monoclonal antibody containing culture supernatant has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure. Following the treatment. the cells were washed x 4. fixed in neutral buffered formalin for 10 min. permeablized and blocked. They were reacted for 60 minutes with the culture supernatant diluted 1:50. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescent microscopy