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D-1 Dopamine Receptor (DRD1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS352066

The antiserum was raised in a rabbit which was immunized with D1(Ac-9-21-Cys22) covalently attached onto a carrier protein. and it has been characterized by immunocytochemical. western immunoblot and ELISA techniques. The antiserum has been found to be highly… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
D-1 Dopamine receptor (DRD1), Polyclonal Antibody
Full Product Name
Rabbit Anti-D1 Receptor Sera
Product Gene Name
anti-DRD1 antibody
Product Synonym Names
D1 Receptor (9-21); D1 Dopamine Receptor
Clonality
Polyclonal
Host
Rabbit
Form/Format
Antiserum Specificity
Specificity
Polypeptide vs % Cross Reactivity
D1 Dopamine Receptor (9-21): 100
D1 Dopamine Receptor: 90
D2 Dopamine Receptor (272-282): 0
D2 Dopamine Receptor: 0
D3 Dopamine Receptor (2-10): 0
D3 Dopamine Receptor: 0
D4 Dopamine Receptor (176-185): 0
D4 Dopamine Receptor: 0
D5 Dopamine Receptor (23-35): 0
D5 Dopamine Receptor: 0
Preparation and Storage
Store at 2 to 8 degree C.
Molecular Weight
49162 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (174867675..174871163, complement). Location: 5q35.1
OMIM
126449
3D Structure
ModBase 3D Structure for P21728

Product Categories/Family for anti-DRD1 antibody

Histochemical Grade Antibodies; Dopamine Receptor Antibodies
Applications Tested/Suitable for anti-DRD1 antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes for anti-DRD1 antibody
Immunocytochemistry: This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections at a dilution of 1:5000. This includes the Medial septum. Nucleus accumbens. Dentate gyrus. Globus pallidus. Medial forebrain bundle. Cortex regions 1-3. Substantia Nigra reticulata. and the Ventral tegmental area.

Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single band being detected at ~50 kD at 1:1000 dilution.

Western Blot Protocol: 1. After SDS-PAGE on 12% gels and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after dilution to at least 1:1000 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.

Other Notes

Small volumes of anti-DRD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DRD1 purchase

MBS352066 is an antibody targeting DRD1. Also known as Rabbit Anti-D1 Receptor Sera. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DRD1. It may not necessarily be applicable to this product.

NCBI GI #
4503383
NCBI GeneID
1812
NCBI Accession #
NP_000785.1
UniProt Primary Accession #
P21728
UniProt Secondary Accession #
Q4QRJ0; B2RA44
UniProt Related Accession #
P21728; Q6FH34
GenBank Nucleotide #
NM_000794.3
NCBI Official Full Name
D(1A) dopamine receptor
NCBI Official Synonym Full Names
dopamine receptor D1
NCBI Official Symbol
DRD1
NCBI Official Synonym Symbols
DADR; DRD1A
UniProt Protein Name
D(1A) dopamine receptor
UniProt Synonym Protein Names
Dopamine D1 receptor
Protein Family
D(1A) dopamine receptor
UniProt Gene Name
DRD1
UniProt Entry Name
DRD1_HUMAN
NCBI Protein Information
D(1A) dopamine receptor; D(1A) dopamine receptor; dopamine D1 receptor

NCBI Summary for DRD1

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq]
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UniProt Comments for DRD1

Function: This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subunit structure: Interacts with DNAJC14 via its C-terminus

By similarity. Interacts with DRD1IP.

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein

By similarity. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14

Tissue specificity: Detected in caudate, nucleus accumbens and in the olfactory tubercle. Ref.1

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.
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Research Articles on DRD1

1. Following a genome wide association study, the authors report a possible association between rs17799914 of DRD1 and schizophrenia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DRD1 antibody

 Reference Product  PubMed Publications
 GRIN1 antibody  >2 publications with DRD1 and GRIN1

Pathways associated with anti-DRD1 antibody

 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Dopamine Receptors Pathway antibodies  Dopamine Receptors Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram

Diseases associated with anti-DRD1 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >75 publications with DRD1 and Mental Disorders
 Schizophrenia Antibodies  >32 publications with DRD1 and Schizophrenia
 Bipolar Disorder Antibodies  >17 publications with DRD1 and Bipolar Disorder
 Dementia Antibodies  >8 publications with DRD1 and Dementia
 Hypertension Antibodies  >7 publications with DRD1 and Hypertension
 Alzheimer Disease Antibodies  >6 publications with DRD1 and Alzheimer Disease
 Drug Toxicity Antibodies  >5 publications with DRD1 and Drug Toxicity
 Psychotic Disorders Antibodies  >4 publications with DRD1 and Psychotic Disorders
 Cognition Disorders Antibodies  >4 publications with DRD1 and Cognition Disorders
 Seizures Antibodies  >2 publications with DRD1 and Seizures

Organs/Tissues associated with anti-DRD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >44 publications with DRD1 and Brain
 Eye Antibodies  >2 publications with DRD1 and Eye
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