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D-4 Dopamine Receptor IgG Fraction (DRD4) Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS352075

The antiserum was raised in a rabbit which was immunized with D4(Ac-176-185) covalently attached onto a carrier protein. and it has been characterized by immunocytochemical. western immunoblot and ELISA techniques. The antiserum and the IgG fraction of the… More details

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Product Overview

Online Datasheet in PDF
Product Name
D-4 Dopamine receptor IgG fraction (DRD4), Polyclonal Antibody
Full Product Name
D-4 Dopamine receptor IgG fraction
Product Gene Name
anti-DRD4 antibody
Product Synonym Names
D 4 (176-185); D 4 Dopamine Receptor; IgG Fraction of Rabbit Anti-D4 Receptor Sera
Clonality
Polyclonal
Form/Format
Antiserum Specificity
Specificity
Polypeptide ::: % Cross Reactivity
D4 Dopamine Receptor (Ac-176-185) ::: 100
D4 Dopamine Receptor ::: 60
Molecular Weight
48,361 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (637305..640706). Location: 11p15.5
OMIM
126452
3D Structure
ModBase 3D Structure for P21917

Product Categories/Family for anti-DRD4 antibody

Histochemical Grade Antibodies; Dopamine Receptor Antibodies
Applications Tested/Suitable for anti-DRD4 antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes for anti-DRD4 antibody
Immunocytochemistry: This IgG fraction has been found to stain specific cells in various regions of PLP fixed rat brain sections at a concentration of 5 ug/ml. This includes the glomeruli of the olfactory bulb. staining of cortex layer 4 and dendritic staining in cortex layers 2 and 3. In layer 5 of the cortex apical dendrites of many neurons were stained. In the hippocampus diffuse immunoreactivity was observed in the molecular layer of the dentate gyrus. with fine dots associated with many neurons in the granule cell layer.

Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single wide band being detected at ~48-53 kD at a concentration of 40 ug/ml.

Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after diluting to 40 ug/ml. Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.

Other Notes

Small volumes of anti-DRD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DRD4 purchase

MBS352075 is an antibody targeting DRD4. Also known as D-4 Dopamine receptor IgG fraction.

Additional Details

NCBI/Uniprot data below describe general gene information for DRD4. It may not necessarily be applicable to this product.

NCBI GI #
1345939
NCBI GeneID
1815
NCBI Accession #
P21917.2
UniProt Primary Accession #
P21917
UniProt Secondary Accession #
Q7Z7Q5; Q8NGM5; B0M0J7
UniProt Related Accession #
P21917; Q0MTB8; Q16303; Q76E76; Q8IZB1; Q8IZB2; Q8IZB3; Q8IZB4; Q8IZB5; Q8IZB6
GenBank Nucleotide #
P21917
NCBI Official Full Name
D(4) dopamine receptor
NCBI Official Synonym Full Names
dopamine receptor D4
NCBI Official Symbol
DRD4
NCBI Official Synonym Symbols
D4DR
UniProt Protein Name
D(4) dopamine receptor
UniProt Synonym Protein Names
D(2C) dopamine receptor; Dopamine D4 receptor
Protein Family
D(4) dopamine receptor
UniProt Gene Name
DRD4
UniProt Entry Name
DRD4_HUMAN
NCBI Protein Information
D(4) dopamine receptor; D(4) dopamine receptor; OTTHUMP00000164435; dopamine D4 receptor; D(2C) dopamine receptor; seven transmembrane helix receptor

NCBI Summary for DRD4

This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. [provided by RefSeq]
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UniProt Comments for DRD4

Function: This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

Subunit structure: Interacts with CLIC6

By similarity and GPRASP1. Ref.9

Subcellular location: Cell membrane; Multi-pass membrane protein.

Polymorphism: The number of repeats of 16 amino acids in the third cytoplasmic loop is highly polymorphic and varies among different alleles. Alleles corresponding in size to a 2 (D4.2), 3 (D4.3), 4 (D4.4), 5 (D4.5), 6 (D4.6), 7 (D4.7) and 9 (D4.9) repeats have been described. The sequence shown is that of allele D4.7. The polymorphic repeat sequence has little influence on DRD4-binding profiles and might not be essential for G protein interaction.

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

Sequence caution: The sequence AAL58637.1 differs from that shown. Reason: Erroneous gene model prediction.
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Research Articles on DRD4

1. The effect of different pooling strategies and genetic models on the reported association between DRD4-exIII-Variable number of tandem repeats polymorphism and cigarette smoking, was investigated.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DRD4 antibody

 Reference Product  PubMed Publications
 SRC antibody  >2 publications with DRD4 and SRC
 GRB2 antibody  >1 publications with DRD4 and GRB2

Pathways associated with anti-DRD4 antibody

 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Dopamine Receptors Pathway antibodies  Dopamine Receptors Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 GPCRs, Other Pathway antibodies  GPCRs, Other Pathway Diagram
 Hypothetical Network For Drug Addiction Pathway antibodies  Hypothetical Network For Drug Addiction Pathway Diagram
 Monoamine GPCRs Pathway antibodies  Monoamine GPCRs Pathway Diagram

Diseases associated with anti-DRD4 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >567 publications with DRD4 and Mental Disorders
 Attention Deficit Disorder with Hyperactivity Antibodies  >259 publications with DRD4 and Attention Deficit Disorder with Hyperactivity
 Attention Deficit and Disruptive Behavior Disorders Antibodies  >218 publications with DRD4 and Attention Deficit and Disruptive Behavior Disorders
 Schizophrenia Antibodies  >184 publications with DRD4 and Schizophrenia
 Substance-Related Disorders Antibodies  >108 publications with DRD4 and Substance-Related Disorders
 Bipolar Disorder Antibodies  >52 publications with DRD4 and Bipolar Disorder
 NOVELTY SEEKING PERSONALITY TRAIT Antibodies  >45 publications with DRD4 and NOVELTY SEEKING PERSONALITY TRAIT
 Anxiety Disorders Antibodies  >36 publications with DRD4 and Anxiety Disorders
 Psychotic Disorders Antibodies  >35 publications with DRD4 and Psychotic Disorders
 Neurodegenerative Diseases Antibodies  >31 publications with DRD4 and Neurodegenerative Diseases

Organs/Tissues associated with anti-DRD4 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >284 publications with DRD4 and Brain
 Ovary Antibodies  >21 publications with DRD4 and Ovary
 Kidney Antibodies  >16 publications with DRD4 and Kidney
 Eye Antibodies  >15 publications with DRD4 and Eye
 Bone Antibodies  >1 publications with DRD4 and Bone
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