Henos [2-12] IgG Fraction Antibody – Polyclonal

The pooled antisera were raised in rabbits which were immunized with heNOS(2-11) covalently attached onto a carrier protein. The pooled antisera have been characterized by western blotting. ELISA. and cell staining techniques using synthetic peptides and recombinant whole proteins. The antibodies are specific for the amino terminal of heNOS. and have been found to be suitable for the immunocytochemical and western blot detection of heNOS. The antibodies recognize the non-myristylated form of heNOS best. but they do cross-react ~50% with the myristylated form of heNOS. The IgG fraction of the pooled rabbit antisera was prepared by precipitation. dialysis. and column chromatography. Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent of whole antisera. or with additional buffer for more dilute antibody.
Each vial contains 2.6 mg of purified IgG.

Small volumes of anti-heNOS [2-12] IgG fraction antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS352104 is an antibody targeting heNOS [2-12] IgG fraction. Reactive in Human.