PKC Zeta IgG Fraction (PRKCZ) Antibody – Rabbit Polyclonal

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Function: This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Phosphatidylinositol 3,4,5-trisphosphate might be a physiological activator. Two specific sites, Thr-410 (activation loop of the kinase domain) and Thr-560 (turn motif), need to be phosphorylated for its full activation.

Subunit structure: Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5

By similarity. Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with ADAP1/CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1

Probable. Interacts with IKBKB. Interacts (via the protein kinase domain) with WWC1. Forms a tripartite complex with WWC1 and DDR1, but predominantly in the absence of collagen. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Ref.7 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16 Ref.19

Subcellular location: Cytoplasm. Endosome. Cell junction. Note: In the retina, localizes in the terminals of the rod bipolar cells

By similarity. Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. Ref.7 Ref.19

Tissue specificity: Expressed in brain, and to a lesser extent in lung, kidney and testis.

Domain: The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B. Ref.14The C1 domain does not bind the diacylglycerol (DAG). Ref.14

Post-translational modification: CDH5 is required for its phosphorylation at Thr-410.

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 OPR domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.

Sequence caution: The sequence CAA78813.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.