• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8200287

MBS8200287 is an antibody targeting CD9. Reactive in Human, Mouse, Rat, Monkey. Host: Rabbit. Rabbit polyclonal antibody to CD9 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD9, Polyclonal Antibody
Full Product Name
Anti-CD9 Antibody
Product Gene Name
anti-CD9 antibody
Product Synonym Names
MIC3; TSPAN29; CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD9
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Monkey
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of CD9 protein.
Preparation and Storage
Upon receipt aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
CD9 peptide (MBS8228615) is used for blocking the activity of CD9 antibody (MBS8200287)
OMIM
143030
3D Structure
ModBase 3D Structure for P21926
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD9. The exact sequence is proprietary.
Applications Tested/Suitable for anti-CD9 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-CD9 antibody
WB: 1/500 - 1/1000
IHC: 1/100 - 1/200
IF/ICC: 1/100 - 1/500

Western Blot (WB) of anti-CD9 antibody

Western blot analysis of CD9 expression in HEK293T (A), NIH3T3 (B), rat heart (C) whole cell lysates.
(Predicted band size: 25 kD; Observed band size: 24 kD)
Rabbit Anti-CD9 Polyclonal Antibody – Cat# MBS8200287

Immunohistochemistry (IHC) of anti-CD9 antibody

Immunohistochemical analysis of CD9 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-CD9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8200287

Immunofluorescence (IF) of anti-CD9 antibody

Immunofluorescent analysis of CD9 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-CD9 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8200287

Other Notes

Small volumes of anti-CD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD9. It may not necessarily be applicable to this product.

NCBI GI #
4502693
NCBI GeneID
928
NCBI Accession #
NP_001760.1
UniProt Primary Accession #
P21926
UniProt Secondary Accession #
Q5J7W6; Q96ES4; D3DUQ9
UniProt Related Accession #
P21926
GenBank Nucleotide #
NM_001769.3
NCBI Official Full Name
CD9 antigen
NCBI Official Synonym Full Names
CD9 molecule
NCBI Official Symbol
CD9
NCBI Official Synonym Symbols
MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
UniProt Protein Name
CD9 antigen
UniProt Synonym Protein Names
5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD_antigen: CD9
Protein Family
CD9 antigen
UniProt Gene Name
CD9
UniProt Synonym Gene Names
MIC3; TSPAN29; MRP-1; Tspan-29
UniProt Entry Name
CD9_HUMAN
NCBI Protein Information
CD9 antigen

NCBI Summary for CD9

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
Show more

UniProt Comments for CD9

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; platelet alpha granule membrane; focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle; external side of plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: platelet activation; negative regulation of cell proliferation; platelet degranulation; fusion of sperm to egg plasma membrane; single fertilization; pathogenesis; brain development; cell motility; blood coagulation; cell adhesion; oligodendrocyte development; response to water deprivation; multicellular organism reproduction; paranodal junction assembly
Show more

Research Articles on CD9

1. Report shows that breast cancer cells contain a nuclear CD9 pool and that the abrogation of CD9 expression results in multipolar mitoses and polynucleation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD9 antibody

 Reference Product  PubMed Publications
 HBEGF antibody  >39 publications with CD9 and HBEGF
 CD36 antibody  >39 publications with CD9 and CD36
 KIT antibody  >24 publications with CD9 and KIT
 ICAM1 antibody  >19 publications with CD9 and ICAM1
 PTGFRN antibody  >12 publications with CD9 and PTGFRN
 ITGA6 antibody  >3 publications with CD9 and ITGA6
 ITGA3 antibody  >2 publications with CD9 and ITGA3

Pathways associated with anti-CD9 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Fertilization Pathway antibodies  Fertilization Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Infectious Disease Pathway antibodies  Infectious Disease Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Reproduction Pathway antibodies  Reproduction Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram

Diseases associated with anti-CD9 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >130 publications with CD9 and Carcinoma
 Neoplasm Metastasis Antibodies  >65 publications with CD9 and Neoplasm Metastasis
 Neoplasms, Germ Cell and Embryonal Antibodies  >58 publications with CD9 and Neoplasms, Germ Cell and Embryonal
 Leukemia, Myeloid, Acute Antibodies  >51 publications with CD9 and Leukemia, Myeloid, Acute
 Inflammation Antibodies  >47 publications with CD9 and Inflammation
 Cardiovascular Diseases Antibodies  >47 publications with CD9 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >39 publications with CD9 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >36 publications with CD9 and Lung Neoplasms
 Breast Neoplasms Antibodies  >34 publications with CD9 and Breast Neoplasms
 Necrosis Antibodies  >33 publications with CD9 and Necrosis

Organs/Tissues associated with anti-CD9 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >470 publications with CD9 and Blood
 Bone Antibodies  >147 publications with CD9 and Bone
 Bone Marrow Antibodies  >129 publications with CD9 and Bone Marrow
 Vascular Antibodies  >92 publications with CD9 and Vascular
 Lung Antibodies  >85 publications with CD9 and Lung
 Embryonic Tissue Antibodies  >68 publications with CD9 and Embryonic Tissue
 Lymph Node Antibodies  >48 publications with CD9 and Lymph Node
 Placenta Antibodies  >46 publications with CD9 and Placenta
 Kidney Antibodies  >45 publications with CD9 and Kidney
 Liver Antibodies  >41 publications with CD9 and Liver
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000