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Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 5, LGR5 (LGR5) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS355156

Leucine-rich repeat-containing G-protein coupled receptor 5(LGR5), also known as GPR49, is a 907-amino acid 7-transmembrane protein, [1] belongs to G Protein-Coupled Receptor. The differential expression of GPR49 between normal and tumor tissue was… More details

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Product Overview

Product Name
Leucine-rich repeat-containing G-protein coupled receptor 5, LGR5, Polyclonal Antibody
Full Product Name
Anti-Leucine-rich repeat-containing G-protein coupled receptor 5, LGR5 antibody
Product Gene Name
anti-LGR5 antibody
Product Synonym Names
Polyclonal Anti- Leucine-rich repeat-containing G-protein coupled receptor 5; LGR5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, mouse
Form/Format
Liquid
Purity/Purification
Immunogen affinity purified
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.
Molecular Weight
99,998 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (71832931..71980090). Location: 12q22-q23
OMIM
606667
3D Structure
ModBase 3D Structure for O75473
Immunogen
A synthetic peptide (conjugated to KLH) corresponding to the region of LGR5.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Applications Tested/Suitable for anti-LGR5 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-LGR5 antibody
WB (1:1000-2000); IHC-P (1:200); ICC (1:50)

Western Blot (WB) of anti-LGR5 antibody

Rabbit Anti-LGR5 Polyclonal Antibody – Cat# MBS355156

Immunohistochemistry (IHC) of anti-LGR5 antibody

Rabbit Anti-LGR5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS355156

Product References and Citations for anti-LGR5 antibody

1. Hsu, S. Y., Liang, S.-G., Hsueh, A. J. W. Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G protein-coupled, seven-transmembrane region. Molec. Endocr. 12: 1830-1845, 1998. 2. McClanahan T, Koseoglu S, Smith K et al. (2007). "Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors". Cancer Biol. Ther. 5 (4): 419-26.

Other Notes

Small volumes of anti-LGR5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LGR5 purchase

MBS355156 is an antibody targeting LGR5. Also known as Anti-Leucine-rich repeat-containing G-protein coupled receptor 5, LGR5 antibody. Reactive in Human, mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LGR5. It may not necessarily be applicable to this product.

NCBI GI #
4504379
NCBI GeneID
8549
NCBI Accession #
NP_003658.1
UniProt Primary Accession #
O75473
UniProt Secondary Accession #
Q4VAM0; Q4VAM2; Q9UP75
UniProt Related Accession #
O75473
GenBank Nucleotide #
NM_003667.3
NCBI Official Full Name
leucine-rich repeat-containing G-protein coupled receptor 5 isoform 1
NCBI Official Synonym Full Names
leucine-rich repeat containing G protein-coupled receptor 5
NCBI Official Symbol
LGR5
NCBI Official Synonym Symbols
FEX; HG38; GPR49; GPR67; GRP49
UniProt Protein Name
Leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Synonym Protein Names
G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
Protein Family
Leucine-rich repeat-containing G-protein coupled receptor
UniProt Gene Name
LGR5
UniProt Synonym Gene Names
GPR49; GPR67
UniProt Entry Name
LGR5_HUMAN
NCBI Protein Information
leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; orphan G protein-coupled receptor HG38; leucine-rich repeat-containing G protein-coupled receptor 5

NCBI Summary for LGR5

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
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UniProt Comments for LGR5

Function: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. Ref.8 Ref.9 Ref.10 Ref.11

Subcellular location: Cell membrane; Multi-pass membrane protein. Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein. Note: Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans-Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (Ref.12). Ref.10 Ref.11 Ref.12

Tissue specificity: Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. Ref.5 Ref.6 Ref.7

Miscellaneous: LGR5 is used as a marker of adult tissue stem cells in the intestine, stomach, hair follicle, and mammary epithelium (Ref.7).

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.Contains 16 LRR (leucine-rich) repeats.Contains 1 LRRNT domain.
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Research Articles on LGR5

1. Both LGR5(+) and LGR5(-) cells were detected in the tumor tissues of colon cancer patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LGR5 antibody

 Reference Product  PubMed Publications
 OLFM4 antibody  >12 publications with LGR5 and OLFM4
 TSPAN8 antibody  >12 publications with LGR5 and TSPAN8
 JAZF1 antibody  >12 publications with LGR5 and JAZF1
 ADAMTS9 antibody  >11 publications with LGR5 and ADAMTS9
 DCLK1 antibody  >11 publications with LGR5 and DCLK1
 THADA antibody  >10 publications with LGR5 and THADA
 CAMK1D antibody  >9 publications with LGR5 and CAMK1D
 CDC123 antibody  >9 publications with LGR5 and CDC123
 MBD3 antibody  >1 publications with LGR5 and MBD3

Diseases associated with anti-LGR5 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >115 publications with LGR5 and Neoplasms
 Colonic Neoplasms Antibodies  >38 publications with LGR5 and Colonic Neoplasms
 Liver Neoplasms Antibodies  >17 publications with LGR5 and Liver Neoplasms
 Inflammation Antibodies  >16 publications with LGR5 and Inflammation
 Liver Diseases Antibodies  >16 publications with LGR5 and Liver Diseases
 Neoplasms, Experimental Antibodies  >11 publications with LGR5 and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >8 publications with LGR5 and Disease Models, Animal
 Precancerous Conditions Antibodies  >8 publications with LGR5 and Precancerous Conditions
 Carcinoma, Hepatocellular Antibodies  >7 publications with LGR5 and Carcinoma, Hepatocellular
 Necrosis Antibodies  >4 publications with LGR5 and Necrosis

Organs/Tissues associated with anti-LGR5 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >141 publications with LGR5 and Intestine
 Liver Antibodies  >37 publications with LGR5 and Liver
 Stomach Antibodies  >30 publications with LGR5 and Stomach
 Skin Antibodies  >21 publications with LGR5 and Skin
 Embryonic Tissue Antibodies  >16 publications with LGR5 and Embryonic Tissue
 Bone Antibodies  >11 publications with LGR5 and Bone
 Muscle Antibodies  >7 publications with LGR5 and Muscle
 Ovary Antibodies  >6 publications with LGR5 and Ovary
 Vascular Antibodies  >6 publications with LGR5 and Vascular
 Brain Antibodies  >5 publications with LGR5 and Brain
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