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Osteopontin, OPN (OPN) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS355195

Osteopontin (OPN), also known as bone sialoprotein I (BSP-1 or BNSP), secreted phosphoprotein 1 (SPP1), is a human gene product. The gene has 7 exons, spans 5 kilobases in length and in humans it is located on 4q13. The protein is composed of ~300 amino acids… More details

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Product Overview

Product Name
Osteopontin, OPN, Polyclonal Antibody
Full Product Name
Anti-Osteopontin, OPN antibody
Product Gene Name
Product Synonym Names
Polyclonal Anti- Osteopontin; OPN
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Liquid
Purity/Purification
Immunogen affinity purified
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles
Molecular Weight
35,423 Da
Chromosome Location
Chromosome: 4; NC_000004.11 (88896802..88904563). Location: 4q22.1
OMIM
166490
3D Structure
ModBase 3D Structure for P10451
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to the region of mouse OPN.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Applications Tested/Suitable for anti-OPN antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-OPN antibody
WB (1:1000); ICC (1:100-200)

Western Blot (WB) of anti-OPN antibody

Rabbit Anti-OPN Polyclonal Antibody – Cat# MBS355195

Product References and Citations for anti-OPN antibody

1. Choi ST, Kim JH, Kang EJ, Lee SW, Park MC, Park YB, Lee SK (December 2008). "Osteopontin might be involved in bone remodelling rather than in inflammation in ankylosing spondylitis". Rheumatology (Oxford) 47 (12): 1775-9. 2. Reinholt FP, Hultenby K, Oldberg A, Heinegard D (June 1990). "Osteopontin--a possible anchor of osteoclasts to bone". Proc. Natl. Acad. Sci. U.S.A. 87 (12): 4473-5.

Other Notes

Small volumes of anti-OPN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OPN purchase

MBS355195 is an antibody targeting OPN. Also known as Anti-Osteopontin, OPN antibody. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for OPN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
osteopontin
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
UniProt Protein Name
Osteopontin
UniProt Synonym Protein Names
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
BNSP; OPN; SPP-1
UniProt Entry Name
OSTP_HUMAN
NCBI Protein Information
osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)

NCBI Summary for OPN

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for OPN

Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity

By similarity.

Subunit structure: Ligand for integrin alpha-V/beta-3.

Subcellular location: Secreted.

Tissue specificity: Bone. Found in plasma.

Post-translational modification: Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. Ref.17 Ref.21N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. Ref.17 Ref.22

Sequence similarities: Belongs to the osteopontin family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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