Enterokinase (Bovine) (TMPRSS15) Enzyme

Name: TMPRSS15 Enzyme; Enterokinase (Bovine); Bovine; MyBioSource
Brand: MyBioSource
SKU: MBS355467
Price: 440 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS355467

Description: Recombinant full length bovine Enterokinase with 6 His tags.Introduction: Recombinant Enterokinase is the light chain of bovine Enterokinase, which is expressed in yeast and purified to yield a high enzyme activity preparation. Enterokinase… More details

$440 / 100 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Enterokinase (Bovine) (TMPRSS15), Enzyme

Full Product Name

Enterokinase (Bovine) (His tag)

Product Gene Name

TMPRSS15 enzyme

Product Synonym Names

Enteropeptidase, Enterokinase, Serine protease 7, ENTK

Host

Pichia pastoris

Form/Format

Lyophilized

Purity/Purification

> 95% by HPLC

Preparation and Storage

Upon receipt, store at -80°C for long term.
Avoid repeated freeze and thaw cycles.

Chromosome Location

Chromosome: 21; NC_000021.8 (19641433..19775970, complement). Location: 21q21.1

OMIM

226200

3D Structure

ModBase 3D Structure for P98073

Reconstitution

Reconstitute with sterile water, then aliquot the reconstituted enzyme at -20°C.

Activity

10,000 U/mg

Unit Definition

One unit is defined as the amount of enzyme needed to cleave 50 ug of control fusion protein for 16 hours at 20°C in the buffer of 20 mM Tris-HCl, 50 mM NaCl, pH 7.2. (The enzyme cleavage condition will be different for different fusion proteins.)

Recommended Cleavage Condition

At 20°C in a buffer with 20 mM Tris-HCl, 50 mM NaCl (pH 7.2).

Provided Assisted Reagents

MBS355467-A: Cleavage Control Protein: 10ug, 1 vial
MBS355467-B: Storage/Reaction Buffer: 2ml (10x), 1 vial
MBS355467-C: Sterile Deionized Water: 1ml, 1 vial

Note: The following protocols are for reference ONLY. The enzyme cleavage condition will be adjusted for different fusion proteins, it is recommended to test several trials to determine the optimal Enterokinase concentration and experimental conditions including temperatures, incubation times, etc.

Example for Enzyme Cleavage

1. Dilute the Enterokinase and Cleavage Control Protein (provided) with cleavage buffer (20mM Tris-HCl, 50mM NaCl, pH 7.2) to the final concentrations of 0.3mg/ml and 1.0g/ml respectively.
2. Mix the related reagents according to the following in a 1.5ml tube:
- Cleavage Buffer (10x): 5ul
- Target Protein: 20ul
- Diluted Enterkinase: 2ul
- Sterile Deionized Water: 23ul
- Total: 50ul
3. Incubate at room temperature (20-21°C for 1h, 3h, 6h, and 24h respectively.
4. Stop the reaction by storing at -20°C.
5. Analyze the cleavage effect of these samples by SDS-PAGE electrophoresis.

Note: The suggested ratio of enzyme and substrate is about 1 U/50ug to 100ug. (The optimal ratio should be determined by end user.)

Usage of Cleavage Control Protein

1. Dilute the Enterokinase with 200ul of Sterile Deionized Water, and Cleavage Control Protein with 15ul of Sterile Deionized Water respectively.
2. Mix the related reagents according to the following in a 1.5ml tube:
- Cleavage Control Protein: 15ul
- Diluted Enterkinase: 2ul
- Storage/Reaction Buffer (1x): 17ul
- Total: 34ul
3. Incubate at room temperature (20-21°C) for 16h.
4. Add 10ul of 2x concentrated SDS-PAGE buffer into 10ul of samples.
5. Boil the samples for 5min and analyse the cleavage effect of these samples by SDS-PAGE electrophoresis.

Product Categories/Family for TMPRSS15 enzyme

Instrumental Enzyme

Other Notes

Small volumes of TMPRSS15 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TMPRSS15 purchase

MBS355467 is an enzyme targeting TMPRSS15. Also known as Enterokinase (Bovine) (His tag). Host: Pichia pastoris.

Additional Details

NCBI/Uniprot data below describe general gene information for TMPRSS15. It may not necessarily be applicable to this product.

NCBI GI #

223942069

NCBI GeneID

5651

NCBI Accession #

NP_002763.2

UniProt Primary Accession #

P98073

UniProt Secondary Accession #

Q2NKL7

UniProt Related Accession #

P98073

GenBank Nucleotide #

NM_002772.2

NCBI Official Full Name

enteropeptidase

NCBI Official Synonym Full Names

transmembrane protease, serine 15

NCBI Official Symbol

TMPRSS15

NCBI Official Synonym Symbols

ENTK; PRSS7

UniProt Protein Name

Enteropeptidase

UniProt Synonym Protein Names

Enterokinase; Serine protease 7; Transmembrane protease serine 15

Protein Family

Enteropeptidase

UniProt Gene Name

TMPRSS15

UniProt Synonym Gene Names

ENTK; PRSS7

UniProt Entry Name

ENTK_HUMAN

NCBI Protein Information

enteropeptidase; enterokinase; serine protease 7; protease, serine, 7 (enterokinase)

NCBI Summary for TMPRSS15

This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008]

UniProt Comments for TMPRSS15

Function: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.

Catalytic activity: Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.

Subunit structure: Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond.

Subcellular location: Membrane; Single-pass type II membrane protein

Probable.

Tissue specificity: Intestinal brush border.

Post-translational modification: The chains are derived from a single precursor that is cleaved by a trypsin-like protease.

Involvement in disease: Enterokinase deficiency (ENTKD) [MIM:226200]: Life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2

Sequence similarities: Belongs to the peptidase S1 family.Contains 2 CUB domains.Contains 2 LDL-receptor class A domains.Contains 1 MAM domain.Contains 1 peptidase S1 domain.Contains 1 SEA domain.Contains 1 SRCR domain.

Sequence caution: The sequence CAB90389.1 differs from that shown. Reason: Erroneous gene model prediction.

Research Articles on TMPRSS15

1. Enteropeptidase is a gene associated with a starvation human phenotype and a novel target for obesity treatment

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.