Paxillin (PAX) ELISA Kit for Human

PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.

Protein type: Cell adhesion; Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; focal adhesion; cytoskeleton; lamellipodium; leading edge; cytoplasm; plasma membrane; stress fiber; cell cortex; nucleus

Molecular Function: integrin binding; zinc ion binding; beta-catenin binding; protein complex binding; BH4 domain binding; protein kinase binding; vinculin binding

Biological Process: focal adhesion formation; lamellipodium biogenesis; integrin-mediated signaling pathway; regulation of cell shape; peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; activation of MAPK activity; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; positive regulation of protein kinase activity; cytoskeleton organization and biogenesis