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Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS036302

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used to determine the level of LPCAT1 (hereafter termed "analyte") in undiluted original Mouse body fluids and tissue homogenates.… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Lysophosphatidylcholine Acyltransferase 1 (LPCAT1), ELISA Kit
Full Product Name
Human Lysophosphatidylcholine Acyltransferase 1 ELISA Kit
Product Gene Name
LPCAT1 elisa kit
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Mouse Body Fluids And Tissue Homogenates
Detection Range
0.625ng/ml-20ng/ml
Sensitivity
0.1ng/ml.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
Intra-assay Precision
Intra-assay CV (%) is less than 15%
Molecular Weight
59,151 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (1461542..1524076, complement). Location: 5p15.33
OMIM
610472
3D Structure
ModBase 3D Structure for Q8NF37

Other Notes

Small volumes of LPCAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LPCAT1 purchase

MBS036302 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LPCAT1. The ELISA analytical biochemical technique of the MBS036302 kit is based on LPCAT1 antibody-LPCAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LPCAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LPCAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LPCAT1. It may not necessarily be applicable to this product.

NCBI GI #
33946291
NCBI GeneID
79888
NCBI Accession #
NP_079106.3
UniProt Primary Accession #
Q8NF37
UniProt Secondary Accession #
Q1HAQ1; Q7Z4G6; Q8N3U7; Q8WUL8; Q9GZW6
UniProt Related Accession #
Q8NF37
GenBank Nucleotide #
NM_024830.3
NCBI Official Full Name
lysophosphatidylcholine acyltransferase 1
NCBI Official Synonym Full Names
lysophosphatidylcholine acyltransferase 1
NCBI Official Symbol
LPCAT1
NCBI Official Synonym Symbols
AYTL2; lpcat; PFAAP3
UniProt Protein Name
Lysophosphatidylcholine acyltransferase 1
UniProt Synonym Protein Names
1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase (EC:2.3.1.67); Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; Acyltransferase-like 2; Phosphonoformate immuno-associated protein 3
Protein Family
Lysophosphatidylcholine acyltransferase
UniProt Gene Name
LPCAT1
UniProt Synonym Gene Names
AYTL2; PFAAP3; LPC acyltransferase 1; LPCAT-1; LysoPC acyltransferase 1; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT
UniProt Entry Name
PCAT1_HUMAN
NCBI Protein Information
lysophosphatidylcholine acyltransferase 1; LPCAT-1; lysoPAFAT; LPC acyltransferase 1; acyltransferase like 2; acyltransferase-like 2; lysoPC acyltransferase 1; lyso-PAF acetyltransferase; regulated by phosphonoformate; acetyl-CoA:lyso-PAF acetyltransferase; phosphonoformate immuno-associated protein 3; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 1; acetyl-CoA:lyso-platelet-activating factor acetyltransferase

NCBI Summary for LPCAT1

Lysophosphatidylcholine (LPC) acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis (Nakanishi et al., 2006 [PubMed 16704971]).[supplied by OMIM, May 2008]

UniProt Comments for LPCAT1

LPCAT1: Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: Membrane protein, integral; EC 2.3.1.67; Endoplasmic reticulum; EC 2.3.1.23; Acetyltransferase

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; lipid particle

Molecular Function: 1-alkylglycerophosphocholine O-acetyltransferase activity; 1-alkenylglycerophosphocholine O-acyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; 1-alkylglycerophosphocholine O-acyltransferase activity; calcium ion binding

Biological Process: retina development in camera-type eye; phospholipid metabolic process; glycerophospholipid biosynthetic process; positive regulation of protein catabolic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; surfactant homeostasis; phospholipid biosynthetic process
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Research Articles on LPCAT1

1. LPCAT1 correlates with the progression of prostate cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LPCAT1 elisa kit

 Reference Product  PubMed Publications
 LPCAT2 elisa kit  >9 publications with LPCAT1 and LPCAT2
 LPCAT4 elisa kit  >2 publications with LPCAT1 and LPCAT4
 PLA2G4A elisa kit  >1 publications with LPCAT1 and PLA2G4A

Pathways associated with LPCAT1 elisa kit

 Products by Pathway  Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Acyl Chain Remodelling Of PG Pathway antibodies  Acyl Chain Remodelling Of PG Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with LPCAT1 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6 publications with LPCAT1 and Inflammation
 Disease Models, Animal Antibodies  >4 publications with LPCAT1 and Disease Models, Animal
 Fatty Liver Antibodies  >3 publications with LPCAT1 and Fatty Liver
 Neoplasms Antibodies  >3 publications with LPCAT1 and Neoplasms
 Nervous System Diseases Antibodies  >2 publications with LPCAT1 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >1 publications with LPCAT1 and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >1 publications with LPCAT1 and Neoplasm Metastasis
 Spinal Cord Diseases Antibodies  >1 publications with LPCAT1 and Spinal Cord Diseases
 Lung Neoplasms Antibodies  >1 publications with LPCAT1 and Lung Neoplasms
 Liver Diseases Antibodies  >1 publications with LPCAT1 and Liver Diseases

Organs/Tissues associated with LPCAT1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >13 publications with LPCAT1 and Lung
 Liver Antibodies  >4 publications with LPCAT1 and Liver
 Blood Antibodies  >3 publications with LPCAT1 and Blood
 Ovary Antibodies  >2 publications with LPCAT1 and Ovary
 Eye Antibodies  >2 publications with LPCAT1 and Eye
 Nerve Antibodies  >1 publications with LPCAT1 and Nerve
 Kidney Antibodies  >1 publications with LPCAT1 and Kidney
 Prostate Antibodies  >1 publications with LPCAT1 and Prostate
 Brain Antibodies  >1 publications with LPCAT1 and Brain
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