Pulmonary Surfactant Associated Protein B (SPB) ELISA Kit for Chicken

Principle of the Assay: SPB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SPB antibody and an SPB-HRP conjugate. The assay sample and buffer are incubated together with SPB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPB concentration since SPB from samples and SPB-HRP conjugate compete for the anti-SPB antibody binding site. Since the number of sites is limited, as more sites are occupied by SPB from the sample, fewer sites are left to bind SPB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPB concentration in each sample is interpolated from this standard curve.

Small volumes of SPB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS740628 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pulmonary Surfactant Associated Protein B (SPB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPB. The ELISA analytical biochemical technique of the MBS740628 kit is based on SPB antibody-SPB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).