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CD34 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS370027

CD34 functions as a cell-cell adhesion factor and cell-surface glycoprotein. It may also mediate the attachment of stem cells to bone marrow extracellular matrixes or directly to stromal cells. Cells expressing CD34 are normally found in the umbilical cord… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD34, Monoclonal Antibody
Full Product Name
CD34
Product Gene Name
anti-CD34 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
QBEnd/10
Host
Mouse
Species Reactivity
Human, Dog
Form/Format
CD34 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Preparation and Storage
Store at 2-8°C (Control Slides: Store at 20-25°C)

Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.
Chromosome Location
Chromosome: 1; NC_000001.10 (208059883..208084683, complement). Location: 1q32
3D Structure
ModBase 3D Structure for Q3C1E7
Immunogen
Human endothelial vesicles.
Control
Tonsil, Placenta, Appendix
Localization
Cytoplasmic, Membranous
Reactivity Note
Paraffin, Frozen
Precautions
1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.
3. Dispose of unused solution with copious amount of water.
4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Laboratories” (see References in this document).
Mounting Protocols
For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter, please inquire.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Application Notes for anti-CD34 antibody
This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations.

Specimen Preparation:
Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate , ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.
Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.

Staining Procedure:
1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Hydrophilic Plus Slides.
2. Air dry for 2 hours at 58°C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.
5. Any of three heating methods may be used:
a. TintoRetriever Pressure Cooker or Equivalent
Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99°C. Incubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with ImmunoDNA washer or DI water.
9. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution.

Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls.

Testing Data of anti-CD34 antibody

Mouse Anti-CD34 Monoclonal Antibody – Cat# MBS370027

Testing Data of anti-CD34 antibody

PRODUCT LIMITATIONS
Mouse Anti-CD34 Monoclonal Antibody, Testing Data – Cat# MBS370027

Testing Data of anti-CD34 antibody

ABBREVIATED IMMUNOHISTOCHEMICAL PROTOCOL
Mouse Anti-CD34 Monoclonal Antibody, Testing Data – Cat# MBS370027

Immunohistochemistry (IHC) of anti-CD34 antibody

IHC of CD34 on a FFPE Kidney Tissue
Mouse Anti-CD34 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS370027

Other Notes

Small volumes of anti-CD34 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD34 purchase

MBS370027 is an antibody targeting CD34. Reactive in Human, Dog. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD34. It may not necessarily be applicable to this product.

NCBI GI #
264769
NCBI GeneID
947
UniProt Primary Accession #
Q3C1E7
UniProt Related Accession #
P28906; Q3C1E7; Q5JTA5; Q7Z375; Q8IUM0; Q9UH97
NCBI Official Full Name
CD34
NCBI Official Synonym Full Names
CD34 molecule
NCBI Official Symbol
CD34
UniProt Protein Name
CD34 antigen
Protein Family
Hematopoietic progenitor cell antigen
UniProt Gene Name
CD34
UniProt Entry Name
Q3C1E7_HUMAN
NCBI Protein Information
hematopoietic progenitor cell antigen CD34; CD34 antigen; OTTHUMP00000034733; OTTHUMP00000034734

NCBI Summary for CD34

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM]

UniProt Comments for CD34

CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: intercellular bridge; integral to plasma membrane; lysosome; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; plasma membrane; basal plasma membrane; external side of plasma membrane

Molecular Function: sulfate binding; carbohydrate binding; transcription factor binding

Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; cell-cell adhesion; positive regulation of angiogenesis; regulation of blood pressure; endothelial cell proliferation; tissue homeostasis; hemopoiesis; leukocyte migration
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Research Articles on CD34

1. stromal cell-derived factor-1-3'A polymorphism has a role in mobilization of hematopoietic stem cells CD34+ in Tunisian population

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD34 antibody

 Reference Product  PubMed Publications
 CRKL antibody  >20 publications with CD34 and CRKL
 PRKCD antibody  >5 publications with CD34 and PRKCD

Pathways associated with anti-CD34 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram

Diseases associated with anti-CD34 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >10307 publications with CD34 and Neoplasms
 Bone Marrow Diseases Antibodies  >2347 publications with CD34 and Bone Marrow Diseases
 Cardiovascular Diseases Antibodies  >1797 publications with CD34 and Cardiovascular Diseases
 Carcinoma Antibodies  >1620 publications with CD34 and Carcinoma
 Skin Diseases Antibodies  >1601 publications with CD34 and Skin Diseases
 Necrosis Antibodies  >1000 publications with CD34 and Necrosis
 Multiple Myeloma Antibodies  >767 publications with CD34 and Multiple Myeloma
 Breast Neoplasms Antibodies  >760 publications with CD34 and Breast Neoplasms
 Adenocarcinoma Antibodies  >759 publications with CD34 and Adenocarcinoma
 Anemia Antibodies  >746 publications with CD34 and Anemia

Organs/Tissues associated with anti-CD34 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >13541 publications with CD34 and Blood
 Bone Antibodies  >7819 publications with CD34 and Bone
 Bone Marrow Antibodies  >7427 publications with CD34 and Bone Marrow
 Vascular Antibodies  >3844 publications with CD34 and Vascular
 Muscle Antibodies  >1854 publications with CD34 and Muscle
 Umbilical Cord Antibodies  >1340 publications with CD34 and Umbilical Cord
 Liver Antibodies  >1265 publications with CD34 and Liver
 Skin Antibodies  >1140 publications with CD34 and Skin
 Lung Antibodies  >800 publications with CD34 and Lung
 Heart Antibodies  >687 publications with CD34 and Heart
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