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Cytokeratin 20 (KRT20) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS370051

Cytokeratin 20 (CK20) is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. Cytokeratin 20 is a Type I cytokeratin. It is a major cellular protein of mature… More details

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Sample Manual Insert View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 20 (KRT20), Monoclonal Antibody
Full Product Name
Cytokeratin 20
Product Gene Name
anti-KRT20 antibody
Clonality
Monoclonal
Isotype
IgG2a/K
Clone Number
Ks20.8
Host
Mouse
Species Reactivity
Human, Dog, Cat, Mouse
Form/Format
Cytokeratin 20 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Preparation and Storage
Store at 2-8°C (Control Slides: Store at 20-25°C)
OMIM
608218
3D Structure
ModBase 3D Structure for P35900
Immunogen
Semi-purified human cytokeratin preparation.
Control
Colon Carcinoma, Colon Mucosa, Bladder
Localization
Cytoplasmic
Reactivity Note
Paraffin, Frozen
Precautions
1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.
3. Dispose of unused solution with copious amount of water.
4. Dispose of unused solution with copious amount of water.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Medical (see References in this document).
Mounting Protocols
For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter, please inquire.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-KRT20 antibody
Immunohistochemistry (IHC)
Application Notes for anti-KRT20 antibody
This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations.

Specimen Preparation:
Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate , ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.
Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.

Staining Procedure:
1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Hydrophilic Plus Slides.
2. Air dry for 2 hours at 58°C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.
5. Any of three heating methods may be used:
a. TintoRetriever Pressure Cooker or Equivalent
Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99°C. Incubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with ImmunoDNA washer or DI water.
9. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution.

Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls.

Dilution Information of anti-KRT20 antibody

Mouse Anti-KRT20 Monoclonal Antibody – Cat# MBS370051

Immunohistochemistry (IHC) of anti-KRT20 antibody

IHC of Cytokeratin 20 on an FFPE Adenocarcinoma Tissue
Mouse Anti-KRT20 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS370051

Abbreviated Immunohistochemical Protocol of anti-KRT20 antibody

Mouse Anti-KRT20 Monoclonal Antibody, Abbreviated Immunohistochemical Protocol – Cat# MBS370051

Performance Characteristics of anti-KRT20 antibody

Mouse Anti-KRT20 Monoclonal Antibody, Performance Characteristics – Cat# MBS370051

Other Notes

Small volumes of anti-KRT20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT20 purchase

MBS370051 is an antibody targeting KRT20. Also known as Cytokeratin 20. Reactive in Human, Dog, Cat, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT20. It may not necessarily be applicable to this product.

NCBI GI #
402645
UniProt Primary Accession #
P35900
UniProt Secondary Accession #
B2R6W7
NCBI Official Full Name
cytokeratin 20
UniProt Protein Name
Keratin, type I cytoskeletal 20
UniProt Synonym Protein Names
Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT
Protein Family
Keratin
UniProt Gene Name
KRT20
UniProt Entry Name
K1C20_HUMAN

UniProt Comments for KRT20

Function: Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine

By similarity. Ref.4 Ref.8

Subunit structure: Heterotetramer of two type I and two type II keratins. Associates with KRT8.

Subcellular location: Cytoplasm Ref.6 Ref.7.

Tissue specificity: Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. Ref.6 Ref.7

Developmental stage: First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. Ref.1

Post-translational modification: Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury

By similarity.Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. Ref.4

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT20 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with KRT20 and FLNA

Pathways associated with anti-KRT20 antibody

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram

Diseases associated with anti-KRT20 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >453 publications with KRT20 and Neoplasms
 Adenocarcinoma Antibodies  >243 publications with KRT20 and Adenocarcinoma
 Colorectal Neoplasms Antibodies  >165 publications with KRT20 and Colorectal Neoplasms
 Neoplasm Metastasis Antibodies  >143 publications with KRT20 and Neoplasm Metastasis
 Urinary Bladder Neoplasms Antibodies  >72 publications with KRT20 and Urinary Bladder Neoplasms
 Endocrine Gland Neoplasms Antibodies  >70 publications with KRT20 and Endocrine Gland Neoplasms
 Colonic Neoplasms Antibodies  >69 publications with KRT20 and Colonic Neoplasms
 Lung Neoplasms Antibodies  >66 publications with KRT20 and Lung Neoplasms
 Skin Neoplasms Antibodies  >56 publications with KRT20 and Skin Neoplasms
 Ovarian Neoplasms Antibodies  >52 publications with KRT20 and Ovarian Neoplasms

Organs/Tissues associated with anti-KRT20 antibody

 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >71 publications with KRT20 and Stomach
 Intestine Antibodies  >40 publications with KRT20 and Intestine
 Kidney Antibodies  >21 publications with KRT20 and Kidney
 Brain Antibodies  >8 publications with KRT20 and Brain
 Muscle Antibodies  >7 publications with KRT20 and Muscle
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