MUC2 Antibody – Mouse Monoclonal

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq]

Function: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Ref.11

Subunit structure: Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization. Interacts with FCGBP. Interacts with AGR2; disulfide-linked. Ref.7 Ref.10 Ref.11

Subcellular location: Secreted. Note: In the intestine, secreted into the inner and outer mucus layers

Tissue specificity: Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.

Post-translational modification: O-glycosylated. Ref.6May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria

By similarity.At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.

Polymorphism: The number of repeats is highly polymorphic and varies among different alleles.

Sequence similarities: Contains 1 CTCK (C-terminal cystine knot-like) domain.Contains 1 TIL (trypsin inhibitory-like) domain.Contains 2 VWFC domains.Contains 4 VWFD domains.