Topoisomerase II Alpha (TOP2A) Antibody – Rabbit Monoclonal

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

TOP2A: a DNA topoisomerase. Controls of topological states of DNA by transient breakage and rejoining of DNA. Makes double-strand breaks. Four splice variant isoforms have been described.

Protein type: Isomerase; EC 5.99.1.3

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: nucleoplasm; centriole; nuclear chromosome; viral integration complex; DNA topoisomerase complex (ATP-hydrolyzing); protein complex; nucleolus; condensed chromosome; nucleus

Molecular Function: protein C-terminus binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA-dependent ATPase activity; ubiquitin binding; protein homodimerization activity; histone deacetylase binding; magnesium ion binding; drug binding; protein binding; enzyme binding; protein kinase C binding; DNA binding; protein heterodimerization activity; chromatin binding; DNA bending activity; ATP binding

Biological Process: DNA unwinding during replication; mitotic recombination; positive regulation of viral genome replication; DNA topological change; positive regulation of apoptosis; sister chromatid segregation; rhythmic process; apoptotic chromosome condensation; regulation of circadian rhythm; embryonic cleavage; chromosome segregation; positive regulation of retroviral genome replication; DNA ligation; resolution of meiotic joint molecules as recombinants; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; mitotic cell cycle; response to DNA damage stimulus