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Annexin A1 (ANXA1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS370183

This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.The… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Annexin A1 (ANXA1), Monoclonal Antibody
Full Product Name
Annexin A1
Product Gene Name
anti-ANXA1 antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
BSB-95
Host
Mouse
Species Reactivity
Paraffin, Frozen
Preparation and Storage
Store at 2-8 °C.

. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.
Chromosome Location
Chromosome: 9; NC_000009.11 (75766721..75785309). Location: 9q21.13
OMIM
151690
3D Structure
ModBase 3D Structure for P04083
Immunogen
Human Annexin A1.
Location
Cytoplasmic, Membranous
Control
Liver, Tonsil, Spleen, Thymus, Lung, Colon, Hairy Cell Leukemia
Staining Procedure
1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate .

5. Any of three heating methods may be used:

a. TintoRetriever r Pressure Cooker or Equivalent
Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA
Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA
Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.

6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with IHC wash buffer or DI water.
9. Continue IHC staining protocol
Precautions
1. For professional users only. Ensure results are interpreted by a medical professional.
2. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, ?ush with large volumes of water to prevent sodium azide build-up.
3. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents.
4. Unused solution should be disposed of according to local and federal regulations.
5. Do not ingest reagent. If reagent ingested, contact a poison control center immediately.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of anti-ANXA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ANXA1 purchase

MBS370183 is an antibody targeting ANXA1. Also known as Annexin A1. Reactive in Paraffin, Frozen. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ANXA1. It may not necessarily be applicable to this product.

NCBI GI #
4502101
NCBI GeneID
301
NCBI Accession #
NP_000691.1
UniProt Primary Accession #
P04083
UniProt Related Accession #
P04083
GenBank Nucleotide #
NM_000700.1
NCBI Official Full Name
annexin A1
NCBI Official Synonym Full Names
annexin A1
NCBI Official Symbol
ANXA1
NCBI Official Synonym Symbols
ANX1; LPC1
UniProt Protein Name
Annexin A1
UniProt Synonym Protein Names
Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
Protein Family
Annexin
UniProt Gene Name
ANXA1
UniProt Synonym Gene Names
ANX1; LPC1
UniProt Entry Name
ANXA1_HUMAN
NCBI Protein Information
annexin A1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein

NCBI Summary for ANXA1

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq, Jul 2008]
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UniProt Comments for ANXA1

Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Subunit structure: Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF

By similarity. Ref.7

Subcellular location: Nucleus

By similarity. Cytoplasm

By similarity. Cell projection › cilium

By similarity. Basolateral cell membrane

By similarity. Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium

By similarity. Found in the cytoplasm of type II pneumocytes and alveolar macrophages

By similarity.

Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.

Post-translational modification: Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. Ref.5 Ref.8

Sequence similarities: Belongs to the annexin family.Contains 4 annexin repeats.
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Research Articles on ANXA1

1. Annexin A1 down regulation in patients with bladder pain syndrome might decrease cell survival and damage urothelial function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ANXA1 antibody

 Reference Product  PubMed Publications
 FPR2 antibody  >20 publications with ANXA1 and FPR2
 POMC antibody  >11 publications with ANXA1 and POMC
 FPR1 antibody  >9 publications with ANXA1 and FPR1
 S100A11 antibody  >5 publications with ANXA1 and S100A11
 FPR3 antibody  >1 publications with ANXA1 and FPR3

Pathways associated with anti-ANXA1 antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway antibodies  Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram

Diseases associated with anti-ANXA1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >86 publications with ANXA1 and Neoplasms
 Inflammation Antibodies  >62 publications with ANXA1 and Inflammation
 Carcinoma Antibodies  >48 publications with ANXA1 and Carcinoma
 Disease Models, Animal Antibodies  >21 publications with ANXA1 and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >16 publications with ANXA1 and Neoplasm Metastasis
 Brain Diseases Antibodies  >15 publications with ANXA1 and Brain Diseases
 Carcinoma, Squamous Cell Antibodies  >13 publications with ANXA1 and Carcinoma, Squamous Cell
 Necrosis Antibodies  >11 publications with ANXA1 and Necrosis
 Pain Antibodies  >9 publications with ANXA1 and Pain
 Fibrosis Antibodies  >8 publications with ANXA1 and Fibrosis

Organs/Tissues associated with anti-ANXA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >75 publications with ANXA1 and Blood
 Brain Antibodies  >24 publications with ANXA1 and Brain
 Lung Antibodies  >18 publications with ANXA1 and Lung
 Vascular Antibodies  >18 publications with ANXA1 and Vascular
 Pituitary Gland Antibodies  >12 publications with ANXA1 and Pituitary Gland
 Liver Antibodies  >11 publications with ANXA1 and Liver
 Lymph Node Antibodies  >10 publications with ANXA1 and Lymph Node
 Muscle Antibodies  >9 publications with ANXA1 and Muscle
 Stomach Antibodies  >8 publications with ANXA1 and Stomach
 Bone Antibodies  >7 publications with ANXA1 and Bone
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