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NeuN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS370226

MBS370226 is an antibody targeting NeuN. Reactive in Human, Mouse, Rat, Chicken, Ferret, Pig. Host: Mouse. More details

$195 / 5 Slides +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NeuN, Monoclonal Antibody
Full Product Name
NeuN
Product Gene Name
anti-NeuN antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
A60
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Chicken, Ferret, Pig
Preparation and Storage
Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.
Intended Use
This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.
Immunogen
Purified cell nuclei from mouse brain.
Summary and Explanation
NeuN (Feminizing Locus on X-3, Fox-3, or Hexaribonucleotide Binding Protein-3) is a neuron-specific protein that is present in most Central Nervous System (CNS) and Peripheral Nervous System (PNS) neuronal cell types. NeuN protein distributions are restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain. However, some neurons fail to be recognized by NeuN at all ages, such as INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells.

NeuN is widely used to label neurons since the vast majority of neurons are strongly positive. NeuN immunoreactivity becomes obvious as neurons mature, typically after they have downregulated expression of Doublecortin, a marker seen in the earliest stages of neuronal development.
Localization
Nuclear
Control
Brain
Presentation
Anti-NeuN is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Precautions
1. For professional users only. Results should be interpreted by a qualified medical professional.
2. This product contains <0.1% sodium azide (NaN?) as a preservative. Ensure proper handling procedures are used with this reagent.
3. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.
4. Dispose of unused solution with copious amount of water.
5. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL”
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.

IHC of anti-NeuN antibody

Inset: IHC of NeuN on a FFPE Brain Tissue
Mouse Anti-NeuN Monoclonal Antibody – Cat# MBS370226

Presentation of anti-NeuN antibody

Mouse Anti-NeuN Monoclonal Antibody, Presentation – Cat# MBS370226

Staining Procedure of anti-NeuN antibody

1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.
5. Any of three heating methods may be used:
a. TintoRetriever Pressure Cooker or Equivalent
Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with IHC wash buffer or DI water.
9. Continue IHC staining protocol.
Mouse Anti-NeuN Monoclonal Antibody, Staining Procedure – Cat# MBS370226

Performance Characteristics of anti-NeuN antibody

Mouse Anti-NeuN Monoclonal Antibody, Performance Characteristics – Cat# MBS370226

Other Notes

Small volumes of anti-NeuN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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