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CD13 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS370280

CD13 (also known as aminopeptidase-N) is expressed on the majority of peripheral blood monocytes and granulocytes. It is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of… More details

$195 / 5 Slides +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD13, Monoclonal Antibody
Full Product Name
CD13
Product Gene Name
anti-CD13 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
38C12
Host
Mouse
Species Reactivity
Human
Form/Format
CD13 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Preparation and Storage
Store at 2 to 8°C.
Stability: This product is stable up to the expiration date on the product label. Do not use after expiration date listed on the package label. Temperature fluctuations should be avoided. Store appropriately when not in use and avoid prolonged exposure to room temperature conditions.
Control
Spleen, Tonsil, Prostate, Liver
Localization
Membranous, Cytoplasmic
Reactivity Note
Paraffin, Frozen
Precautions
1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
2. Always wear personal protective equipment such as a laboratorycoat, goggles, and gloves when handling reagents.
3.Dispose of unused solution with copious amounts of water.
4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document,“Guidelines for Safe Work Practices in Human and AMDL”.
Mounting Protocols
For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter.
Product Limitations
Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized, and incubation time), optimal performance should be established through the use of positive and negative controls.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Application Notes for anti-CD13 antibody
Specimen Preparation:
Paraffin sections: The antibodycan be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using Bio SB ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA, or ImmunoDNA Digestor. Tissue should remain hydrated via use of Bio SB Immuno/DNAWasher solutions.

Frozen sections and cell preparations:
The antibodycan be used on acetone-fixed frozen sections and acetone-fixed cell preparations.

IHC Protocol:
1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positivelycharged slides such as Bio SB Hydrophilic Plus Slides
2. Air dryfor 2 hours at 58°C.
3. Deparaffinize, dehydrate, and rehydrate tissues.
4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.
5. Any of three heating methods may be used:
a.TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b.TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C.Incubate for 30-60 minutes.
c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual IHC, perform antibodyincubation at ambient temperature.For automated IHC methods, perform antibodyincubation according to instrument manufacturer’s instructions.
8.Wash slides with ImmunoDNA washer or DI water.
9. Continue IHC protocol.Wash slides between each step with ImmunoDNA washer solution.

Dilution Information of anti-CD13 antibody

Mouse Anti-CD13 Monoclonal Antibody – Cat# MBS370280

Immunohistochemistry (IHC) of anti-CD13 antibody

Mouse Anti-CD13 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS370280

Immunohistochemistry (IHC) of anti-CD13 antibody

Inset: IHC of CD13 on a FFPE Tonsil Tissue
Mouse Anti-CD13 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS370280

Testing Data (TD) of anti-CD13 antibody

Mouse Anti-CD13 Monoclonal Antibody, Testing Data (TD) – Cat# MBS370280

Other Notes

Small volumes of anti-CD13 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD13 purchase

MBS370280 is an antibody targeting CD13. Reactive in Human. Host: Mouse.
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