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CD68 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS370295

This antibody is intended for use in immunohistochemical applications on formalin fixed paraffin- embedded tissues (FFPE), frozen tissue sections and cell preparations.The CD68 antigen is a heavily glycosylated trans-membrane protein of 87-115 kDa which is… More details

$245 / 3 mL (RTU) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD68, Monoclonal Antibody
Full Product Name
CD68
Product Gene Name
anti-CD68 antibody
Clonality
Monoclonal
Isotype
IgG1/κ
Clone Number
KP-1
Host
Mouse
Species Reactivity
Human, Hamster, Mouse, Non-Human Primate, Porcine, Rabbit, Rat (Reactivity: Paraffin, Frozen)
Form/Format
Anti-CD68 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Preparation and Storage
Store at 2-8°C.
OMIM
153634
Immunogen
Subcellular fraction of human alveolar macrophages.
Localization
Cytoplasmic, Membranous
Control
Tonsil, Lymph Node
Intended Use
This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations.
Applications
Leukemia & Histiocytic, Kidney & Urothelial Cancer,Breast Cancer
Precautions
  1. For professional users only. Results should be interpreted by a qualified medical professional.
  2. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
  3. Always wear personal protective equipment such as a laboratory coat, goggles, and gloves when handling reagents.
  4. Dispose of unused solution with copious amounts of water.
  5. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
  6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
  7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
  8. For additional safety information refer to Safety Data Sheet for this product.
  9. For complete recommendations for handling biological specimens, please refer to the CDC document, "Guidelines for Safe Work Practices in Human and AMDL" (see References in this document).
Mounting Protocols
For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter please contact Technical Support.
Stability
This product is stable up to the expiration date on the product label. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.
Product Limitations
Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-CD68 antibody
Immunohistochemistry (IHC)
Application Notes for anti-CD68 antibody
Specimen Preparation
Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA, or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.
Frozen sections and cell preparations: The antibody can be used on acetone-fixed frozen sections and acetone-fixed cell preparations.
IHC Protocol:
  1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Hydrophilic Plus Slides (Please Inquire).
  2. Air dry for 2 hours at 58°C.
  3. Deparaffinize, dehydrate, and rehydrate tissues.
  4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (Please inquire) or EDTA (Please inquire).
  5. Any of three heating methods may be used:
  1. TintoRetriever Pressure Cooker or Equivalent: Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
  2. TintoRetriever PT Module or Water Bath Method: Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
  3. Conventional Steamer Method: Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.
  1. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
  2. For manual IHC, perform antibody incubation at ambient temperature. For automated IHC methods, perform antibody incubation according to instrument manufacturer's instructions.
  3. Wash slides with ImmunoDNA washer or DI water.
  4. Continue IHC protocol. Wash slides between each step with ImmunoDNA washer solution.

Dilution Information of anti-CD68 antibody

Mouse Anti-CD68 Monoclonal Antibody – Cat# MBS370295

Immunohistochemistry (IHC) of anti-CD68 antibody

Inset: IHC of CD68 on a FFPE Lymphoblastic Lymphoma Tissue
Mouse Anti-CD68 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS370295

Abbreviated Immunohistochemical Protocol of anti-CD68 antibody

Recommended IHC Protocol
Mouse Anti-CD68 Monoclonal Antibody, Abbreviated Immunohistochemical Protocol – Cat# MBS370295

Other Notes

Small volumes of anti-CD68 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD68 purchase

MBS370295 is an antibody targeting CD68. Reactive in Human, Hamster, Mouse, Non-Human Primate, Porcine, Rabbit, Rat (Reactivity: Paraffin, Frozen). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD68. It may not necessarily be applicable to this product.

NCBI GI #
298665
NCBI GeneID
968
NCBI Accession #
AAB25811.1
UniProt Secondary Accession #
Q53HR6; Q53XI3; Q96BI7; B4DVT4
UniProt Related Accession #
P34810
NCBI Official Full Name
CD68
NCBI Official Synonym Full Names
CD68 molecule
NCBI Official Symbol
CD68
NCBI Official Synonym Symbols
GP110; LAMP4; SCARD1
UniProt Protein Name
Macrosialin
UniProt Synonym Protein Names
Gp110; CD_antigen: CD68
Protein Family
Macrosialin
UniProt Gene Name
CD68
UniProt Entry Name
CD68_HUMAN
NCBI Protein Information
macrosialin

NCBI Summary for CD68

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD68

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: endosome membrane; integral to membrane; lysosomal membrane; membrane; plasma membrane
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Research Articles on CD68

1. Microenvironment CD20 + cells seem to play a favorable prognostic role in classical Hodgkin Lymphoma. Depletion of CD20 + cells together with an increase of TAMs identifies a group of patients with high-risk disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD68 antibody

 Reference Product  PubMed Publications
 CD34 antibody  >692 publications with CD68 and CD34
 CD163 antibody  >575 publications with CD68 and CD163
 CD36 antibody  >103 publications with CD68 and CD36
 PTPRC antibody  >31 publications with CD68 and PTPRC
 ITGAM antibody  >21 publications with CD68 and ITGAM
 EMR1 antibody  >17 publications with CD68 and EMR1
 ITGAX antibody  >4 publications with CD68 and ITGAX
 EMR2 antibody  >1 publications with CD68 and EMR2
 CD8A antibody  >1 publications with CD68 and CD8A

Pathways associated with anti-CD68 antibody

 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram

Diseases associated with anti-CD68 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1982 publications with CD68 and Inflammation
 Cardiovascular Diseases Antibodies  >1066 publications with CD68 and Cardiovascular Diseases
 Necrosis Antibodies  >1010 publications with CD68 and Necrosis
 Disease Models, Animal Antibodies  >587 publications with CD68 and Disease Models, Animal
 Fibrosis Antibodies  >560 publications with CD68 and Fibrosis
 Lung Diseases Antibodies  >492 publications with CD68 and Lung Diseases
 Kidney Diseases Antibodies  >426 publications with CD68 and Kidney Diseases
 Atherosclerosis Antibodies  >415 publications with CD68 and Atherosclerosis
 Liver Diseases Antibodies  >354 publications with CD68 and Liver Diseases
 Adenocarcinoma Antibodies  >351 publications with CD68 and Adenocarcinoma

Organs/Tissues associated with anti-CD68 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3159 publications with CD68 and Blood
 Vascular Antibodies  >1552 publications with CD68 and Vascular
 Muscle Antibodies  >1194 publications with CD68 and Muscle
 Skin Antibodies  >1005 publications with CD68 and Skin
 Bone Antibodies  >967 publications with CD68 and Bone
 Brain Antibodies  >920 publications with CD68 and Brain
 Liver Antibodies  >623 publications with CD68 and Liver
 Lymph Node Antibodies  >596 publications with CD68 and Lymph Node
 Lung Antibodies  >562 publications with CD68 and Lung
 Kidney Antibodies  >562 publications with CD68 and Kidney
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