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Cytokeratin 3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375045

MBS375045 is an antibody targeting Cytokeratin 3. Also known as Mouse Anti-Cytokeratin 3 [AE5] Antibody. Reactive in Rabbit, Cow, Human. Host: Mouse. More details

$635 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 3, Monoclonal Antibody
Full Product Name
Mouse Anti-Cytokeratin 3 [AE5] Antibody
Product Synonym Names
CK3 antibody; FLJ95909 antibody; K3 antibody; 65 kDa cytokeratin antibody; CK 3 antibody; Cytokeratin3 antibody; Keratin 3 antibody; Keratin type II cytoskeletal 3 antibody; Keratin3 antibody; KRT 3 antibody; KRT3 antibody;
Clonality
Monoclonal
Isotype
Ig
Clone Number
AE5
Host
Mouse
Species Reactivity
Rabbit, Cow, Human
Form/Format
Purified antibody containing PBS 0.1% sodium azide;
Purity/Purification
Protein G purified
Concentration
0.7mg/ml (lot specific)
Target Antigen
Cytokeratin 3
Immunogen
Rabbit corneal epithelial keratins
Myeloma/fusion Partners
P3 x 63 Ag8 myeloma cells
Research Areas
Cell Biology
Applications Tested/Suitable for anti-Cytokeratin 3 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-Cytokeratin 3 antibody
Optimal antibody dilution should be determined by titration, however as guideline try at WB: 1/1000 - 1/3000. Predicted molecular weight: 65 kDa. Flow Cyt: Use 2ug for 106 cells.

Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-Cytokeratin 3 antibody

Mouse Anti-Cytokeratin 3 Monoclonal Antibody – Cat# MBS375045

Product References and Citations for anti-Cytokeratin 3 antibody

Shahdadfar A et al. Ex vivo expanded autologous limbal epithelial cells on amniotic membrane using a culture medium with human serum as single supplement.Exp Eye Res. 2012 Apr;97(1):1-9. doi: 10.1016/j.exer.2012.01.013. Epub 2012 Feb 7. IHC ; Human PMID: 22342952 Hanson C et al. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta Ophthalmol : (2012) PMID: 22280565 Priya CG et al. Adult human buccal epithelial stem cells: identification, ex-vivo expansion, and transplantation for corneal surface reconstruction.Eye (Lond). 2011 Dec;25(12):1641-9. doi: 10.1038/eye.2011.230. Epub 2011 Sep 23.IHC ; Human PMID: 21941360 Liao H et al. Development of Allele-Specific Therapeutic siRNA in Meesmann Epithelial Corneal Dystrophy. PLoS One 6:e28582 (2011). ICC/IF ; Human PMID: 22174841 Utheim TP et al. Comparison of the histology, gene expression profile, and phenotype of cultured human limbal epithelial cells from different limbal regions.Invest Ophthalmol Vis Sci. 2009 Nov;50(11):5165-72. doi: 10.1167/iovs.08-2884. Epub 2009 Jul 2.IHC ; Human PMID: 19578011 Raeder S et al. Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.Invest Ophthalmol Vis Sci. 2007 Dec;48(12):5484-93.IHC ; Human PMID: 18055796 Raeder S et al. Effect of limbal explant orientation on the histology, phenotype, ultrastructure and barrier function of cultured limbal epithelial cells.Acta Ophthalmol Scand. 2007 Jun;85(4):377-86. Epub 2007 Jan 31. IHC ; Human PMID: 17263847 Rodrigues M et al. Suprabasal expression of a 64-kilodalton keratin (no. 3) in developing human corneal epithelium. Differentiation 34:60-7 (1987) PMID: 2440750 Cooper D & Sun TT Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression. J Biol Chem 261:4646-54PMID:2420789 Schermer A et al. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 103:49-62 (1986) PMID: 2424919

Other Notes

Small volumes of anti-Cytokeratin 3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Cytokeratin 3. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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