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Lamin A+c Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375085

MBS375085 is an antibody targeting Lamin A+C. Also known as Mouse Anti-Lamin A+C [JOL2]. Host: Mouse. More details

$360 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Lamin A+C, Monoclonal Antibody
Full Product Name
Mouse Anti-Lamin A+C [JOL2]
Product Synonym Names
70 kDa Lamin antibody; Cardiomyopathy dilated 1A (autosomal dominant) antibody; CDCD1 antibody; CDDCantibody; Charcot Marie Tooth Disease Axonal Type 2B1 antibody; CMD1A antibody; CMT2B1 antibody; EMD2antibody; FPL antibody; FPLD antibody; HGPS antibody; IDC antibody; Lamin A/C antibody; Lamin A/C Isoform 1Precursor antibody; Lamin A/C Isoform 2 antibody; Lamin A/C Isoform 3 antibody; LDP1 antibody; LFPantibody; LGMD1B antibody; Limb girdle muscular dystrophy 1B (autosomal dominant) antibody; LMN 1 antibody; LMN Aantibody; LMN C antibody; LMN1 antibody; LMNA antibody; LMNC antibody; NY REN 32 antigen antibody; PRO1antibody; Progeria 1 (Hutchinson Gilford Type) antibody; Renal carcinoma antigen NY REN 32 antibody; Renalcarcinoma antigen NYREN32 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
JOL2
Host
Mouse
Form/Format
Unconcentrated Tissue Culture Supernatant (RPMI + 10% FCS) plus 0.09% sodium azide
Purity/Purification
Unpurified
Specificity
The antibody reacts with both recombinant and native lamin A and C in humans. It has been shown to bind to an epitope between amino acids 464-572
Preparation and Storage
Store at +4°C for one month, or in small aliquots at -20°C for longer periods. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody
Matching Pairs
Unconjugated Azide Free Antibody: Lamin A+C Clone #JOL2 (MBS375085)
HRP Conjugated Antibody: Lamin A+C Clone #JOL2 (MBS375342)
Immunogen
Recombinant human lamin A
Myeloma/fusion Partners
Spleen cells from immunised BALB/C mice were fused with cells of the mouse). SP2-0/Ag4 myeloma cell line.
Applications Tested/Suitable for anti-Lamin A+C antibody
Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-Lamin A+C antibody
Optimal antibody dilution should be determined by titration, however as a guideline use at: WB 1:1000, IF 1:100, Flow cytometry 1:1000

Immunofluorescence (IF) of anti-Lamin A+C antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear membrane and nucleoplasm staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.
Mouse Anti-Lamin A+c Monoclonal Antibody – Cat# MBS375085

Flow Cytometry (FC/FACS) of anti-Lamin A+C antibody

Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control
Mouse Anti-Lamin A+c Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS375085

Testing Data of anti-Lamin A+C antibody

(1:1000) staining in A431(A), (1:10000) staining in HeLa(B), and (1:1000) staining in HepG2(C) cells nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Mouse Anti-Lamin A+c Monoclonal Antibody, Testing Data – Cat# MBS375085

Immunohistochemistry (IHC) of anti-Lamin A+C antibody

IHC using Lamin A/C Antibody (IQ332) clone JOL2 on Human skin
Mouse Anti-Lamin A+c Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS375085

Immunohistochemistry (IHC) of anti-Lamin A+C antibody

IHC using Lamin A/C antibody (IQ332) clone JOL2 on Human colon
Mouse Anti-Lamin A+c Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS375085

Immunohistochemistry (IHC) of anti-Lamin A+C antibody

Lamin A+C Monoclonal Antibody [JOL2]
Mouse Anti-Lamin A+c Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS375085

Immunofluorescence (IF) of anti-Lamin A+C antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear membrane and nucleoplasm staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.
Mouse Anti-Lamin A+c Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS375085

Flow Cytometry (FC/FACS) of anti-Lamin A+C antibody

Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control
Mouse Anti-Lamin A+c Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS375085

Product References and Citations for anti-Lamin A+C antibody

Both emerin and lamin C depend on lamin A for localization at the nuclear envelope. Vaughan A (J Cell Sci,Jul 2001)PUBMED Iglesias-Guimarais V et al. Apoptotic DNA Degradation into Oligonucleosomal Fragments, but Not Apoptotic Nuclear Morphology, Relies on a Cytosolic Pool of DFF40/CAD Endonuclease. J Biol Chem 287:7766-79 (2012). WB ; HumanPUBMED Harrigan JA et al. Replication stress induces 53BP1-containing OPT domains in G1 cells. J Cell Biol 193:97-108 (2011). ICC/IF ; HumanPUBMED Gudise S et al. Samp1 is functionally associated with the LINC complex and A-type lamina networks. J Cell Sci 124:2077-85 (2011)PUBMED Harten IA et al. Age-Dependent Loss of MMP-3 in Hutchinson-Gilford Progeria Syndrome. J Gerontol A Biol Sci Med Sci : (2011)PUBMED Toulany M et al. Impact of oncogenic K-RAS on YB-1 phosphorylation induced by ionizing radiation. Breast Cancer Res 13:R28 (2011). WB ; HumanPUBMED Mamchaoui K et al. Immortalized pathological human myoblasts: towards a universal tool for the study of neuromuscular disorders. Skelet Muscle 1:34 (2011). ICC/IF ; HumanPUBMED Negroni E et al. In vivo myogenic potential of human CD133+ muscle-derived stem cells: a quantitative study. Mol Ther 17:1771-8 (2009). ICC/IF ; HumanPUBMED

Other Notes

Small volumes of anti-Lamin A+C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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