Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear membrane and nucleoplasm staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.
Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control
(1:1000) staining in A431(A), (1:10000) staining in HeLa(B), and (1:1000) staining in HepG2(C) cells nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IHC using Lamin A/C Antibody (IQ332) clone JOL2 on Human skin
IHC using Lamin A/C antibody (IQ332) clone JOL2 on Human colon
Lamin A+C Monoclonal Antibody [JOL2]
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear membrane and nucleoplasm staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.
Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control