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Trichohyalin (TCHH) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375127

MBS375127 is an antibody targeting TCHH. Also known as Mouse Anti-Trichohyalin [AE15]. Reactive in Mouse, Human. Host: Mouse. More details

$545 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Trichohyalin (TCHH), Monoclonal Antibody
Full Product Name
Mouse Anti-Trichohyalin [AE15]
Product Gene Name
Product Synonym Names
220K trichohyalin granular protein antibody; Hair follicle protein AHFantibody; MGC157889 antibody; MGC157890 antibody; RP11-107M16.3 antibody; TCHH antibody; THH antibody; THLantibody; Trichohyalin granular protein antibody
Clonality
Monoclonal
Isotype
IgG
Clone Number
AE15
Host
Mouse
Species Reactivity
Mouse, Human
Form/Format
Purified antibody containing PBS 0.1% sodium azide;
Purity/Purification
Protein G
Concentration
1mg/ml (lot specific)
Specificity
This antibody recognises a 220K trichohyalin granular protein, a marker for hair follicular differentiation
Molecular Weight
253,925 Da
OMIM
190370
3D Structure
ModBase 3D Structure for Q07283
Target Antigen
Trichohyalin
Immunogen
Urea-mercaptoethanol extract of human hair follicles
Myeloma/fusion Partners
PAI
Research Areas
Calcium ion binding; Cytokeratins; Cytoskeleton
Applications Tested/Suitable for anti-TCHH antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes for anti-TCHH antibody
Optimal antibody dilution should be determined by titration however as a guideline try; WB: Use at an assay dependent dilution. Predicted molecular weight: 220 kDa. IHC-Fr: Use at an assay dependent dilution. PubMed: 19786578 IHC-P: Use at an assay dependent dilution. PubMed: 19038788

Immunohistochemistry (IHC) of anti-TCHH antibody

Mouse Anti-TCHH Monoclonal Antibody – Cat# MBS375127

Product References and Citations for anti-TCHH antibody

Properties and histochemical application of a novel antibody against trichohyalin granules. Takahashi et al (Arch Dermatol Res,2007) PUBMED Larouche D et al. Tissue-engineered skin preserving the potential of epithelial cells to differentiate into hair after grafting. Tissue Eng Part A 17:819-30 (2011). IHC-Fr ; Mouse PUBMED Youssef KK et al. Identification of the cell lineage at the origin of basal cell carcinoma. Nat Cell Biol : (2010). IHC-Fr ; Mouse PUBMED Van Keymeulen A et al. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis. J Cell Biol 187:91-100 (2009). ChIP/Chip, IHC-Fr ; Mouse PUBMED Okubo T et al. Cell Lineage Mapping of Taste Bud Cells and Keratinocytes in the Mouse Tongue and Soft Palate. Stem Cells : (2008). IHC-P ; Mouse PUBMED

Other Notes

Small volumes of anti-TCHH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TCHH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
trichohyalin
NCBI Official Synonym Full Names
trichohyalin
NCBI Official Symbol
TCHH
NCBI Official Synonym Symbols
THH; THL; TRHY
UniProt Protein Name
Trichohyalin
Protein Family
UniProt Gene Name
TCHH
UniProt Synonym Gene Names
THH; THL; TRHY
UniProt Entry Name
TRHY_HUMAN
NCBI Protein Information
trichohyalin

NCBI Summary for TCHH

Trichohyalin confers mechanical strength to the hair follicle inner root sheath and to other toughened epithelial tissues, such as the hard palate and filiform ridges of the tongue, by forming multiple complex crosslinks with itself and with other structural proteins (Steinert et al., 2003 [PubMed 12853460]).[supplied by OMIM, Dec 2009]
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UniProt Comments for TCHH

THH: Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation. Belongs to the S100-fused protein family.

Protein type: Calcium-binding; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoskeleton

Molecular Function: calcium ion binding

Biological Process: keratinization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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