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Histone H3 (H3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375166

MBS375166 is an antibody targeting H3. Also known as Mouse Anti-Histone H3 (tri methyl K9, di methyl K9) [6F12-H4]. Reactive in Reacts with Human and cow. predicted to detect many other species due to sequence identity such as Mouse, Rat, Rabbit, Hamster,… More details

$635 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Histone H3, Monoclonal Antibody
Full Product Name
Mouse Anti-Histone H3 (tri methyl K9, di methyl K9) [6F12-H4]
Product Gene Name
Product Synonym Names
Histone H3.1 antibody; FLJ92264 antibody; H3 histone antibody; H3 histone family, member A antibody; H3/a antibody; H3/b antibody; H3/c antibody; H3/d antibody; h3/f antibody; H3/h antibody; H3/i antibody; H3/j antibody; H3/k antibody; H3/l antibody; H31_HUMAN antibody; H3F1K antibody; H3F3 antibody; H3FA antibody; H3FB antibody; H3FC antibody; H3FD antibody; H3FF antibody; H3FH antibody; H3FI antibody; H3FJ antibody; H3FK antibody; H3FL antibody; HIST1H3A antibody; HIST1H3B antibody; HIST1H3C antibody; HIST1H3D antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6F12-H4
Host
Mouse
Species Reactivity
Reacts with Human and cow. predicted to detect many other species due to sequence identity such as Mouse, Rat, Rabbit, Hamster, Pig, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster), Non Human Primates
Form/Format
Purified antibody (from supernatant) containing PBS + 0.1% sodium azide
Purity/Purification
Protein G
Concentration
1mg/ml (lot specific)
Specificity
Specific for K9 tri-methylated histone H3; weak cross-reactivity with K9 di- and even less monomethylated histone H3 but not unmethylated K9
Molecular Weight
15,406 Da
Target Antigen
Histone H3 (tri methyl K9, di methyl K9)
Immunogen
Synthetic peptide of Human Histone H3, tri methylated at K9
Myeloma/fusion Partners
X63-Ag8.653
Applications Tested/Suitable for anti-H3 antibody
Dot Blot (DB), Western Blot (WB) against endogenous protein, ELISA (EIA), ChIP
Application Notes for anti-H3 antibody
Optimal antibody dilution should be determined by titration, however as a guideline try up to 1:1000 for western blot.Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Histone H3 peptide - tri methyl K9

Western Blot (WB) of anti-H3 antibody

Mouse Anti-H3 Monoclonal Antibody – Cat# MBS375166

Other Notes

Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
histone H3
UniProt Protein Name
Histone H3.3
Protein Family
UniProt Gene Name
H3
UniProt Entry Name
H33_TOBAC

UniProt Comments for H3

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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