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Lamin B2 (LMNB2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375205

MBS375205 is an antibody targeting LMNB2. Also known as Mouse Anti-Lamin B2 [LN43] - Nuclear Envelope Marker. Reactive in Mouse, Hamster, Human, Xenopus laevis, Zebrafish. Host: Mouse. More details

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Product Overview

Product Name
Lamin B2 (LMNB2), Monoclonal Antibody
Full Product Name
Mouse Anti-Lamin B2 [LN43] - Nuclear Envelope Marker
Product Gene Name
Product Synonym Names
Lamin B1 antibody; Lamin B2 antibody; LMN antibody; LMN B2 antibody; LMN2 antibody; LMNBantibody; LMNB1 antibody; LMNB2 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LN43
Host
Mouse
Species Reactivity
Mouse, Hamster, Human, Xenopus laevis, Zebrafish
Form/Format
Purified antibody in PBS containing 0.09% sodium azide
Concentration
1mg/ml (lot specific)
Specificity
Reacts with an epitope located in the C-terminal part of lamin B2.
Molecular Weight
67,689 Da
OMIM
150341
3D Structure
ModBase 3D Structure for Q03252
Target Antigen
Lamin B2 - Nuclear Envelope Marker
Immunogen
Detergent insoluble fraction of potoroo cell line PtK1.
Myeloma/fusion Partners
Mouse myeloma cells with spleen cells from a mouse
Research Areas
Apoptosis; Cell Biology; Chromatin; Class V Lamins; Cytoskeleton; Cytoskeleton / ECM; Intermediate Filaments; Lamins; Nucleoplasm; Nucleus; Subcellular Markers; Tags Cell Markers
Applications Tested/Suitable for anti-LMNB2 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin, Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-LMNB2 antibody
Optimal antibody dilution should be determined by titration, however as a guideline try; ICC: 1/400. Flow Cyt: Use 1ug for 106 cells. IHC-Fr: 1/1 - 1/1. (when used with an avidin-biotinylated HRP complex as detection agent). WB: 1/100 - 1/1000. Detects a band of approximately 65-67 kDa.

Immunohistochemistry (IHC) of anti-LMNB2 antibody

Mouse Anti-LMNB2 Monoclonal Antibody – Cat# MBS375205

Product References and Citations for anti-LMNB2 antibody

Tsai YJ et al. Ribosome distribution in HeLa cells during the cell cycle. PLoS One 7:e32820 (2012). ICC/IF ; Human PUBMED Shimi T et al. The role of nuclear lamin B1 in cell proliferation and senescence. Genes Dev 25:2579-93 (2011) PUBMED Ishimoto K et al. Sterol-mediated Regulation of Human Lipin 1 Gene Expression in Hepatoblastoma Cells. J Biol Chem 284:22195-205 (2009). WB ; Human PUBMED Mackay DR et al. The nucleoporin Nup153 has separable roles in both early mitotic progression and the resolution of mitosis. Mol Biol Cell 20:1652-60 (2009). WB ; Human PUBMED Taimen P et al. A progeria mutation reveals functions for lamin A in nuclear assembly, architecture, and chromosome organization. Proc Natl Acad Sci U S A : (2009). WB ; Human PUBMED Hisatsune J et al. Molecular Characterization of Helicobacter pylori VacA Induction of IL-8 in U937 Cells Reveals a Prominent Role for p38MAPK in Activating Transcription Factor-2, cAMP Response Element Binding Protein, and NF-{kappa}B Activation. J Immunol 180:5017-27 (2008). WB ; Human PUBMED Koutsami MK et al. Centrosome abnormalities are frequently observed in non-small-cell lung cancer and are associated with aneuploidy and cyclin E overexpression. J Pathol 209:512-21 (2006) PUBMED Tsai MY et al. A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311:1887-93 (2006). ICC/IF ; Human PUBMED Broers JL et al. A- and B-type lamins are differentially expressed in normal human tissues. Histochem Cell Biol 107:505-17 (1997) PUBMED Krohne G. Lamins. Methods Cell Biol. 2004;78:573-96WB, ICC/IF, IP. Mouse, HumanPMID: 15646632`

Other Notes

Small volumes of anti-LMNB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LMNB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
lamin B2, partial
NCBI Official Synonym Full Names
lamin B2
NCBI Official Symbol
LMNB2
NCBI Official Synonym Symbols
LMN2; LAMB2
UniProt Protein Name
Lamin-B2
Protein Family
UniProt Gene Name
LMNB2
UniProt Synonym Gene Names
LMN2
UniProt Entry Name
LMNB2_HUMAN
NCBI Protein Information
lamin-B2; lamin B3

NCBI Summary for LMNB2

This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy. [provided by RefSeq, May 2012]
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UniProt Comments for LMNB2

Lamin B2: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Lamin B2 may be lost during the follicle centre stage of B-cell differentiation in humans. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: lamin filament; nuclear inner membrane

Molecular Function: structural molecule activity

Disease: Lipodystrophy, Partial, Acquired, Susceptibility To
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LMNB2 antibody

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