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Gemin 3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375230

MBS375230 is an antibody targeting Gemin 3. Also known as Mouse Anti-Gemin 3 [12H12]. Reactive in Human, Rat, Mouse, Hamster. Host: Mouse. More details

$545 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Gemin 3, Monoclonal Antibody
Full Product Name
Mouse Anti-Gemin 3 [12H12]
Product Synonym Names
Component of gems 3 antibody; DDX 20 antibody; DDX20 antibody; DDX20_HUMAN antibody; DEAD (Asp Glu AlaAsp) box polypeptide 20 antibody; DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 antibody; DEAD box protein 20antibody; DEAD box protein DP 103 antibody; DEAD box protein DP103 antibody; DEAD-box protein DP103antibody; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody; DEAD/H (Asp Glu Ala Asp/His) boxpolypeptide 20 antibody; DEAD/H BOX 20 antibody; DKFZP434H052 antibody; DP 103 antibody; DP103 antibody; Gemin-3antibody; Gemin3 antibody; Probable ATP dependent RNA helicase DDX20 antibody; Probable ATP-dependent RNA helicaseDDX20 antibody; SMN interacting protein antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
12H12
Host
Mouse
Species Reactivity
Human, Rat, Mouse, Hamster
Form/Format
Purified antibody (from supernatant) containing PBS 0.1% sodium azide;
Purity/Purification
Protein A
Concentration
1mg/ml (lot specific)
Target Antigen
Gemin 3
Immunogen
Recombinant 6His-tag COOH-terminal domain of Gemin3 (amino acids 368-548)
Myeloma/fusion Partners
SP2/0
Research Areas
Neuroscience; signal transduction; Splicing
Applications Tested/Suitable for anti-Gemin 3 antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-Gemin 3 antibody
Optimal antibody dilution should be determined by titration, however as a guideline try: WB: Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa). Flow Cyt: Use 1ug for 106 cells. ICC/IF: 1/50.

Immunohistochemistry (IHC) of anti-Gemin 3 antibody

Mouse Anti-Gemin 3 Monoclonal Antibody – Cat# MBS375230

Product References and Citations for anti-Gemin 3 antibody

Mahmoudi S et al. WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies. PLoS Biol 8:e1000521 (2010). WB ; HumanPUBMED Bradrick SS & Gromeier M Identification of gemin5 as a novel 7-methylguanosine cap-binding protein. PLoS One 4:e7030 (2009). WB ; HumanPUBMED Castell³ A et al. RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage. J Cell Sci 122:3799-809 (2009). WB ; HumanPUBMED Walker MP et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; HamsterPUBMED Morency E et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; HumanPUBMED Murashov AK et al. RNAi pathway is functional in peripheral nerve axons. FASEB J. 2007 Mar;21(3):656-70. Epub 2007 Jan 5.ICC/IF, IHC, WB; Rat MousePMID: 17209129 Hamamoto S et al. Identification of a novel human immunodeficiency virus type 1 integrase interactor, Gemin2, that facilitates efficient viral cDNA synthesis in vivo. J Virol. 2006 Jun;80(12):5670-7.WB; HumanPMID: 16731905 Zhang H et al. Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons. J Neurosci 26:8622-32 (2006)PUBMED Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. Charroux et.al (J Cell Biol,Dec 1999)PUBMED

Other Notes

Small volumes of anti-Gemin 3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Gemin 3. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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