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hnRNP A1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375248

MBS375248 is an antibody targeting hnRNP A1. Also known as Mouse Anti-hnRNP A1 [9H10]. Reactive in Mouse, Human. Host: Mouse. More details

$545 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
hnRNP A1, Monoclonal Antibody
Full Product Name
Mouse Anti-hnRNP A1 [9H10]
Product Gene Name
Product Synonym Names
HNRNPA 1 antibody; Helix destabilizing protein antibody; Helix-destabilizing proteinantibody; Heterogeneous nuclear ribonucleoprotein A1 antibody; Heterogeneous nuclear ribonucleoprotein A1B proteinantibody; Heterogeneous nuclear ribonucleoprotein B2 protein antibody; Heterogeneous nuclear ribonucleoprotein coreprotein A1 antibody; hnRNP A1 antibody; hnRNP core protein A1 antibody; HNRNPA1 antibody; HNRPA1antibody; MGC102835 antibody; Nuclear ribonucleoprotein particle A1 protein antibody; ROA1_HUMANantibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9H10
Host
Mouse
Species Reactivity
Mouse, Human
Form/Format
Purified antibody (from supernatant) containing PBS 0.1% sodium azide;
Purity/Purification
Protein A
Concentration
1mg/ml (lot specific)
Target Antigen
hnRNP A1
Immunogen
Full length hnRNP-A1 native protein (partially purified) from HeLa cells (human)
Myeloma/fusion Partners
Balb/c Sp2/0 cell line
Research Areas
RNA binding; RNA Processing
Applications Tested/Suitable for anti-hnRNP A1 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-hnRNP A1 antibody
Optimal antibody dilution should be determined by titration, however as a guideline try; Flow Cyt: Use 1ug for 106 cells. IHC-P: Use a concentration of 1 ug/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol WB: Use at 1:2000-1:4000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa) IP: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex

Immunohistochemistry (IHC) of anti-hnRNP A1 antibody

Mouse Anti-hnRNP A1 Monoclonal Antibody – Cat# MBS375248

Product References and Citations for anti-hnRNP A1 antibody

RNA binding specificity of hnRNP A1: significance of hnRNP A1 highaffinity binding sites in premRNA splicing. Burd CG (Embo J,Mar 1994) PUBMED Kashima T et al. hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing. Hum Mol Genet. 2007 Dec 15;16(24):3149-59. Epub 2007 Sep 19. WB, IP; HumanPMID: 17884807 Woolley AG et al. Prognostic Association of YB-1 Expression in Breast Cancers: A Matter of Antibody. PLoS One 6:e20603 (2011). ICC/IF ; Human PUBMED Kocgozlu L et al. Selective and uncoupled role of substrate elasticity in the regulation of replication and transcription in epithelial cells. J Cell Sci 123:29-39 (2010). ICC/IF ; Marsupials PUBMED Rosenberger S et al. Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation. Proc Natl Acad Sci U S A : (2010). WB ; Human PUBMED Sellier C et al. Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients. EMBO J 29:1248-61 (2010) PUBMED Read EK & Digard P Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export. J Gen Virol 91:1290-301 (2010). WB, In Situ Hybridisation ; Human PUBMED Beauchamp P et al. The cleavage of HuR interferes with its transportin-2-mediated nuclear import and promotes muscle fiber formation. Cell Death Differ 17:1588-99 (2010). WB ; Mouse PUBMED Lieberthal JG et al. The role of YY1 in reduced HP1alpha gene expression in invasive human breast cancer cells. Breast Cancer Res 11:R42 (2009). WB ; Human PUBMED van der Giessen K & Gallouzi IE Involvement of transportin 2-mediated HuR import in muscle cell differentiation. Mol Biol Cell 18:2619-29 (2007). WB, ICC/IF ; Mouse PUBMED

Other Notes

Small volumes of anti-hnRNP A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for hnRNP A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
hnRNP A1, partial

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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