FBXW7/CDC4 Antibody – Mouse Monoclonal

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

FBXW7: a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins including MYC, cyclin E, NOTCH1-released notch intracellular domain (NICD), JUN, and probably PSEN1. Recognizes and binds to phosphorylated target proteins. Other components of the SCF(FBXW7) complex are sCUL1, RBX1, and SKP1A. Defects in FBXW7 may be a cause of breast cancer. The homolog of Cdc4, one of the yeast genes that regulate sister chromatid cohesion. Down-regulation or disruption of FBXW7 resulted in abnormal sister chromatid cohesion in human cells. Four alternatively spliced isoforms have been described.

Protein type: Nucleolus; Motility/polarity/chemotaxis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: nucleoplasm; Golgi apparatus; protein complex; endoplasmic reticulum; cytoplasm; nucleolus; SCF ubiquitin ligase complex

Molecular Function: protein binding, bridging; identical protein binding; protein binding; cyclin binding; ubiquitin protein ligase binding; transcription factor activity

Biological Process: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; viral reproduction; protein stabilization; sister chromatid cohesion; protein ubiquitination; lipid homeostasis; negative regulation of DNA endoreduplication; regulation of protein localization; regulation of transcription, DNA-dependent; vasculature development; positive regulation of epidermal growth factor receptor activity; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; negative regulation of Notch signaling pathway; vasculogenesis; response to DNA damage stimulus; lung development