Phosphofructokinase- Platelet (PFKP) ELISA Kit for Mouse

Name: Mouse PFKP ELISA Kit; Phosphofructokinase- Platelet; MyBioSource
Brand: MyBioSource
SKU: MBS037558
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS037558

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of PFKP (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Phosphofructokinase- Platelet (PFKP), ELISA Kit

Full Product Name

Mouse Phosphofructokinase- Platelet ELISA Kit

Product Gene Name

PFKP elisa kit

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Detection Range

0.625ng/ml-20ng/ml

Sensitivity

0.1ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

85,596 Da

Chromosome Location

Chromosome: 10; NC_000010.10 (3109712..3178997). Location: 10p15.3-p15.2

OMIM

171840

3D Structure

ModBase 3D Structure for Q01813

Other Notes

Small volumes of PFKP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PFKP purchase

MBS037558 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphofructokinase- Platelet (PFKP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PFKP. The ELISA analytical biochemical technique of the MBS037558 kit is based on PFKP antibody-PFKP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFKP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PFKP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PFKP. It may not necessarily be applicable to this product.

NCBI GI #

71297291

NCBI GeneID

5214

NCBI Accession #

AAH43156.1

UniProt Primary Accession #

Q01813

UniProt Secondary Accession #

Q5VSR7; Q5VSR8; B3KS15

UniProt Related Accession #

Q01813

NCBI Official Full Name

PFKP protein

NCBI Official Synonym Full Names

phosphofructokinase, platelet

NCBI Official Symbol

PFKP

NCBI Official Synonym Symbols

PFKF; PFK-C

UniProt Protein Name

6-phosphofructokinase type C

UniProt Synonym Protein Names

6-phosphofructokinase, platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase

Protein Family

ATP-dependent 6-phosphofructokinase

UniProt Gene Name

PFKP

UniProt Synonym Gene Names

PFKF; PFK-C

UniProt Entry Name

K6PP_HUMAN

NCBI Protein Information

6-phosphofructokinase type C; phosphohexokinase; phosphofructokinase 1; phosphofructo-1-kinase isozyme C; Phosphofructokinase, platelet type; 6-phosphofructokinase, platelet type

NCBI Summary for PFKP

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008]

UniProt Comments for PFKP

PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.

Protein type: Kinase, other; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 10p15.3-p15.2

Cellular Component: membrane; nucleus; cytosol

Molecular Function: metal ion binding; protein complex binding; 6-phosphofructokinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process

Research Articles on PFKP

1. KLF4 plays a role in maintenance of high glycolytic metabolism by transcriptional activation of the PFKP gene in breast cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PFKP elisa kit

Reference Product	PubMed Publications
ALDOA elisa kit	>2 publications with PFKP and ALDOA
PFKL elisa kit	>1 publications with PFKP and PFKL
PFKFB3 elisa kit	>1 publications with PFKP and PFKFB3
HK2 elisa kit	>1 publications with PFKP and HK2
PFKM elisa kit	>1 publications with PFKP and PFKM

Pathways associated with PFKP elisa kit

Products by Pathway	Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway antibodies	Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram

Diseases associated with PFKP elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>3 publications with PFKP and Neoplasms
Disease Models, Animal Antibodies	>2 publications with PFKP and Disease Models, Animal
Hypertrophy Antibodies	>1 publications with PFKP and Hypertrophy
Neoplasms, Experimental Antibodies	>1 publications with PFKP and Neoplasms, Experimental
Hypertension Antibodies	>1 publications with PFKP and Hypertension
Breast Neoplasms Antibodies	>1 publications with PFKP and Breast Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with PFKP and Carcinoma, Hepatocellular

Organs/Tissues associated with PFKP elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with PFKP and Blood
Muscle Antibodies	>4 publications with PFKP and Muscle
Ovary Antibodies	>4 publications with PFKP and Ovary
Liver Antibodies	>3 publications with PFKP and Liver
Bone Antibodies	>2 publications with PFKP and Bone
Thyroid Antibodies	>2 publications with PFKP and Thyroid
Brain Antibodies	>2 publications with PFKP and Brain
Heart Antibodies	>1 publications with PFKP and Heart
Skin Antibodies	>1 publications with PFKP and Skin