• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PARP1 Enzyme Human (PARP1) Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS376020

Background: Poly-ADP-ribose metabolism plays a mayor role in a wide range of biological processes, such as maintenance of genomic stability, transcriptional regulation, energy metabolism and apoptosis. At least six poly-ADP-ribose polymerase (PARP) mammalian… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
PARP1 Enzyme human, Protein
Full Product Name
PARP1 Enzyme human, lyophilized
Form/Format
50 ug PARP1 enzyme, human, lyophilized. When reconstituted with 100 uL H2O, concentration will be 0.5 mg/mL (see below for reconstitution instructions).
Purity/Purification
Purity >95% by SDS-PAGE.
Preparation and Storage
Stable for 6 months from date of shipment when stored desiccated at 4 degree C, or more stable at -70 degree C.
Reconstitution
Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open vial and add 100 uL dH2O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate. Upon reconstitution with 100 uL dH20, the final concentrations are as follows: 0.5 mg/mL PARP1 enzyme, 20 mM Tris, pH8, 0.3 M NaCl, 0.1 mM EDTA, 1 mM DTT, plus lyophilization stabilizers. Store reconstituted PARP1 enzyme at -70 degree C. CAUTION: There is loss of PARP enzymatic activity upon each free/thaw cycle. It is suggested to aliquot the reconstituted enzyme into multiple tubes and freeze at -70 degree C. Alternatively, add glycerol at 1:1 vol/vol to the reconstituted PARP1, mix gently by trituration, store at -20 degree C (do not store in a frost-free freezer!) for up to 6 months.
Applications Tested/Suitable for PARP1 Enzyme human protein
Western Blot (WB), SDS-PAGE standard, ELISA (EIA)
Application Notes for PARP1 Enzyme human protein
Enzyme activity/inhibition assays Western Blot, 100 ng/well using HRP/TMB; <20 ng/well for ECL. SDS-PAGE standard, CB stained, 1 ug per well ELISA, 10 ng/well
To the reconstituted PARP1 enzyme, prepared as above, add an equal volume of SDS-PAGE 2x sample buffer containing reducing agent (betaME or DTT). Vortex, heat at 90 degree C for 2 minutes, then spin briefly. Store at -20 or -70 degree C.
1000 U/vial, specific activity=20000 U/mg PARP1. 1U=10 fmol ADP-ribose incorporated into 5 ug immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values.

Testing Data of PARP1 Enzyme human protein

PARP1 Protein – Cat# MBS376020

Testing Data #2 of PARP1 Enzyme human protein

PARP1 Protein, Testing Data #2 – Cat# MBS376020

Testing Data #3 of PARP1 Enzyme human protein

PARP1 Protein, Testing Data #3 – Cat# MBS376020

Product References and Citations for PARP1 Enzyme human protein

D. Lamarre et al. (1988) Biochim. Biophys. Acta 950 147 Y.A. Lazebnik et al. (1994) Nature (Lond.) 371 346 P.J. Duriez et al. (1997) Biochim. Biophys. Acta 1334 65 F.R. Sallmann et al. (1997) Biochem. Cell Biol. 75 451 B.C. Woodhouse and G.L. Dianov (2008) DNA Repair (Amst.) 7 1077 W.L. Kraus (2008) Curr. Opin. Cell Biol. 20 294 P.O. Hassa and M.O. Hottiger (2008) Frontiers Bioscience 13 3046
Y. Zhang et al. (2013) Nature Methods 10, 981

Other Notes

Small volumes of PARP1 Enzyme human protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PARP1 Enzyme human purchase

MBS376020 is a protein targeting PARP1 Enzyme human. Also known as PARP1 Enzyme human, lyophilized.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000