Ganglioside M1 (GM1) ELISA Kit for Human

Principle of the Assay: The kit uses a double?antigen sandwich enzyme?linked immunosorbent assay (ELISA) to analyze the existence or not of Human Anti-GM1 in samples. Add sample to wells pre?coated with one Human Ganglioside M1, at same time add HRP?conjugated Human Ganglioside M1 to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human Anti-GM1 in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.

Small volumes of GM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS037799 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ganglioside M1 Antibody (GM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GM1. The ELISA analytical biochemical technique of the MBS037799 kit is based on GM1 antibody-GM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).