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TGF Beta1 (TGFB1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9600620

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TGF beta1 (TGFB1), Polyclonal Antibody
Full Product Name
TGF beta1 Antibody
Product Gene Name
anti-TGFB1 antibody
Product Synonym Names
Cartilage-inducing factor; CED; Differentiation inhibiting factor; DPD1; LAP; Latency-associated peptide; Prepro transforming growth factor beta 1; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1; Transforming Growth Factor-beta1
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Concentration
~1mg/ml (lot specific)
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
TGF beta1 peptide (MBS9615175) is used for blocking the activity of TGF beta1 antibody (MBS9600620)
Molecular Weight
44~65kd (precursor)
28kD(dimer), 15kD(monomer)
3D Structure
ModBase 3D Structure for P01137
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human TGF beta1,corresponding to a region within C-terminal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TGFB1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-TGFB1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:200
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

Western Blot (WB) of anti-TGFB1 antibody

Western blot analysis of extracts from 3T3, using TGF beta1 Ab. The lane on the left was treated with blocking peptide.
Rabbit Anti-TGFB1 Polyclonal Antibody – Cat# MBS9600620

Immunohistochemistry (IHC) of anti-TGFB1 antibody

MBS9600620 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.
Rabbit Anti-TGFB1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9600620

Immunofluorescence (IF) of anti-TGFB1 antibody

MBS9600620 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9600620 1:200) and mouse anti-beta tubulin Ab( MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).
Rabbit Anti-TGFB1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9600620

Product References and Citations for anti-TGFB1 antibody

Zhao J, Wu M, Chen S, Ji Z, Zheng X; Journal: Neurourol Urodyn. TGF-beta1 and connexin-43 expression in neurogenic bladder from rats with sacral spinal cord injury. Hui Z, Dingjie X, Yuan Y, Zhongqiu W, Na M, Mingjian B, Yu G, Guangyuan L, Xuemin G, Shifeng L, Yucong G, Fang Y, Summer R, Hong X; Journal: Toxicol Appl Pharmacol. Silicosis decreases bone mineral density in rats. Xia Y, Deng J, Zhou Q, Shao X, Yang X, Sha M, Zou H; Journal: Transpl Immunol. Expression and significance of Sirt1 in renal allografts at the early stage of chronic renal allograft dysfunction. Yan C, Li B, Fan F, Du Y, Ma R, Cheng XD, Li XY, Zhang B, Yu Q, Wang YG, Tang RX, Zheng KY; Journal: Sci Rep. The roles of Toll-like receptor 4 in the pathogenesis of pathogen-associated biliary fibrosis caused by Clonorchis sinensis. Ai XY, Liu HJ, Lu C, Liang CL, Sun Y, Chen S, Sun B, Li Y, Liu YR, Zhang Q, Liu XQ, Xiao T, Jing XS, Sun T, Zhou HG, Yang C; Journal: Theranostics. Phenytoin silver: a new nanocompound for promoting dermal wound healing via comprehensive pharmacological action.

Other Notes

Small volumes of anti-TGFB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TGFB1 purchase

MBS9600620 is an antibody targeting TGFB1. Also known as TGF beta1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TGFB1. It may not necessarily be applicable to this product.

NCBI GI #
63025222
NCBI GeneID
7040
NCBI Accession #
NP_000651.3
UniProt Primary Accession #
P01137
UniProt Secondary Accession #
Q9UCG4; A8K792
UniProt Related Accession #
P01137
GenBank Nucleotide #
NM_000660.6
NCBI Official Full Name
transforming growth factor beta-1 proprotein preproprotein
NCBI Official Synonym Full Names
transforming growth factor beta 1
NCBI Official Symbol
TGFB1
NCBI Official Synonym Symbols
CED; LAP; DPD1; TGFB; TGFbeta
UniProt Protein Name
Transforming growth factor beta-1 proprotein
Protein Family
Transforming growth factor
UniProt Gene Name
TGFB1
UniProt Synonym Gene Names
LAP; TGF-beta-1
NCBI Protein Information
transforming growth factor beta-1 proprotein; transforming growth factor beta-1

NCBI Summary for TGFB1

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]
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UniProt Comments for TGFB1

Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.

Research Articles on TGFB1

1. FXR agonist treatment enhanced TGF-beta-induced epithelial mesenchymal transition(EMT) morphologic changes and FXR antagonist inhibited the effect of TGF-beta;. Thus, FXR activation enhances EMT in hepatocellular carcinoma (HCC) and FXR antagonists may be EMT-suppressing drug candidates.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TGFB1 antibody

 Reference Product  PubMed Publications
 TGFB2 antibody  >983 publications with TGFB1 and TGFB2
 CTGF antibody  >559 publications with TGFB1 and CTGF
 ENG antibody  >282 publications with TGFB1 and ENG
 BMP2 antibody  >205 publications with TGFB1 and BMP2
 THBS1 antibody  >144 publications with TGFB1 and THBS1
 MMP2 antibody  >107 publications with TGFB1 and MMP2
 TGFBR3 antibody  >86 publications with TGFB1 and TGFBR3
 DCN antibody  >80 publications with TGFB1 and DCN
 SPARC antibody  >60 publications with TGFB1 and SPARC
 TGFBR2 antibody  >53 publications with TGFB1 and TGFBR2

Pathways associated with anti-TGFB1 antibody

 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with anti-TGFB1 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >4145 publications with TGFB1 and Fibrosis
 Lung Diseases Antibodies  >1277 publications with TGFB1 and Lung Diseases
 Liver Cirrhosis Antibodies  >723 publications with TGFB1 and Liver Cirrhosis
 Hypertension Antibodies  >626 publications with TGFB1 and Hypertension
 Pulmonary Fibrosis Antibodies  >623 publications with TGFB1 and Pulmonary Fibrosis
 Proteinuria Antibodies  >460 publications with TGFB1 and Proteinuria
 Diabetic Nephropathies Antibodies  >406 publications with TGFB1 and Diabetic Nephropathies
 Hypersensitivity Antibodies  >390 publications with TGFB1 and Hypersensitivity
 Asthma Antibodies  >354 publications with TGFB1 and Asthma
 Diabetes Mellitus, Experimental Antibodies  >321 publications with TGFB1 and Diabetes Mellitus, Experimental

Organs/Tissues associated with anti-TGFB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4785 publications with TGFB1 and Blood
 Muscle Antibodies  >2835 publications with TGFB1 and Muscle
 Kidney Antibodies  >2462 publications with TGFB1 and Kidney
 Bone Antibodies  >2130 publications with TGFB1 and Bone
 Lung Antibodies  >1764 publications with TGFB1 and Lung
 Liver Antibodies  >1721 publications with TGFB1 and Liver
 Connective Tissue Antibodies  >1545 publications with TGFB1 and Connective Tissue
 Skin Antibodies  >1075 publications with TGFB1 and Skin
 Heart Antibodies  >1068 publications with TGFB1 and Heart
 Bone Marrow Antibodies  >709 publications with TGFB1 and Bone Marrow
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