Leukotriene C4 (LTC4) ELISA Kit for Fish

Name: Fish LTC4 ELISA Kit; Leukotriene C4; MyBioSource
Brand: MyBioSource
SKU: MBS038303
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS038303

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated LTC4 concentrations in Fish serum, plasma and other body fluids. Using Purified Fish LTC4 antibody to coat Microelisa Stripplate wells… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Product Name

Leukotriene C4 (LTC4), ELISA Kit

Full Product Name

Fish Leukotriene C4 ELISA Kit

Product Gene Name

LTC4 elisa kit

Species Reactivity

Fish

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

31.2pg/ml-1000pg/ml.

Sensitivity

5.0pg/ml.

Specificity

No significant cross-reactivity or interference between Fish LTC4 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

16,567 Da

OMIM

246530

3D Structure

ModBase 3D Structure for Q16873

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated LTC4 concentrations in Fish serum, plasma and other body fluids. Using Purified Fish LTC4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LTC4 and LTC4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LTC4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of LTC4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LTC4 purchase

MBS038303 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leukotriene C4 (LTC4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LTC4. The ELISA analytical biochemical technique of the MBS038303 kit is based on LTC4 antibody-LTC4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LTC4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LTC4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LTC4. It may not necessarily be applicable to this product.

NCBI GI #

22035629

NCBI GeneID

4056

NCBI Accession #

NP_665874.1

UniProt Primary Accession #

Q16873

UniProt Secondary Accession #

Q8N6P0; Q9UC73; Q9UD18

UniProt Related Accession #

Q16873

GenBank Nucleotide #

NM_145867.1

NCBI Official Full Name

leukotriene C4 synthase

NCBI Official Synonym Full Names

leukotriene C4 synthase

NCBI Official Symbol

LTC4S

UniProt Protein Name

Leukotriene C4 synthase

UniProt Synonym Protein Names

Leukotriene-C(4) synthase

Protein Family

Leukotriene C4 synthase

UniProt Gene Name

LTC4S

UniProt Synonym Gene Names

LTC4 synthase

UniProt Entry Name

LTC4S_HUMAN

NCBI Protein Information

leukotriene C4 synthase; LTC4 synthase

NCBI Summary for LTC4

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family includes a number of human proteins, several of which are involved the production of leukotrienes. This gene encodes an enzyme that catalyzes the first step in the biosynthesis of cysteinyl leukotrienes, potent biological compounds derived from arachidonic acid. Leukotrienes have been implicated as mediators of anaphylaxis and inflammatory conditions such as human bronchial asthma. This protein localizes to the nuclear envelope and adjacent endoplasmic reticulum. [provided by RefSeq, Jul 2008]

UniProt Comments for LTC4

LTC4S: Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly. Belongs to the MAPEG family.

Protein type: EC 4.4.1.20; Nuclear envelope; Lyase; Lipid Metabolism - arachidonic acid; Membrane protein, integral; Membrane protein, multi-pass; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; nuclear envelope

Molecular Function: protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; glutathione peroxidase activity; lipid binding

Biological Process: leukotriene biosynthetic process; positive regulation of catalytic activity; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Leukotriene C4 Synthase Deficiency

Research Articles on LTC4

1. Biochemical and structural characterization of LTC4S mutants along with crystal structures of the wild type and mutated enzymes in complex with three product analogs, provide new insights to binding of substrates and product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LTC4 elisa kit

Reference Product	PubMed Publications
ALOX5 elisa kit	>27 publications with LTC4 and ALOX5
ALOX5AP elisa kit	>13 publications with LTC4 and ALOX5AP
LTA4H elisa kit	>13 publications with LTC4 and LTA4H
ABCC1 elisa kit	>4 publications with LTC4 and ABCC1
MGST1 elisa kit	>1 publications with LTC4 and MGST1

Diseases associated with LTC4 elisa kit

Disease Name	Pubmed Publications
Asthma Antibodies	>59 publications with LTC4 and Asthma
Respiratory Hypersensitivity Antibodies	>41 publications with LTC4 and Respiratory Hypersensitivity
Inflammation Antibodies	>21 publications with LTC4 and Inflammation
Asthma, Aspirin-Induced Antibodies	>15 publications with LTC4 and Asthma, Aspirin-Induced
Drug Hypersensitivity Antibodies	>11 publications with LTC4 and Drug Hypersensitivity
Rhinitis Antibodies	>7 publications with LTC4 and Rhinitis
Fibrosis Antibodies	>4 publications with LTC4 and Fibrosis
Necrosis Antibodies	>4 publications with LTC4 and Necrosis
Eosinophilia Antibodies	>3 publications with LTC4 and Eosinophilia
Hyperalgesia Antibodies	>2 publications with LTC4 and Hyperalgesia

Organs/Tissues associated with LTC4 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>14 publications with LTC4 and Lung
Bone Antibodies	>6 publications with LTC4 and Bone
Brain Antibodies	>3 publications with LTC4 and Brain
Ovary Antibodies	>1 publications with LTC4 and Ovary