Sterol Regulatory Element Binding Proteins (SREBP) ELISA Kit for Rat

Name: Rat SREBP ELISA Kit; Sterol Regulatory Element Binding Proteins; MyBioSource
Brand: MyBioSource
SKU: MBS038511
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS038511

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used to determine the level of SREBP (hereafter termed "analyte") in undiluted original Mouse body fluids and tissue homogenates.… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Sterol Regulatory Element Binding Proteins (SREBP), ELISA Kit

Full Product Name

Rat Sterol Regulatory Element Binding Proteins ELISA Kit

Product Gene Name

SREBP elisa kit

Species Reactivity

Rat

Assay Type

Quantitative Sandwich

Samples

Mouse Body Fluids And Tissue Homogenates

Detection Range

31.2pg/ml-1000pg/ml

Sensitivity

5.0pg/ml.

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%

Molecular Weight

123,688 Da

OMIM

600481

3D Structure

ModBase 3D Structure for Q12772

Other Notes

Small volumes of SREBP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SREBP purchase

MBS038511 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sterol Regulatory Element Binding Proteins (SREBP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SREBP. The ELISA analytical biochemical technique of the MBS038511 kit is based on SREBP antibody-SREBP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SREBP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SREBP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SREBP. It may not necessarily be applicable to this product.

NCBI GI #

451330

NCBI GeneID

6721

NCBI Accession #

AAA50746.1

UniProt Primary Accession #

Q12772

UniProt Secondary Accession #

Q05BD5; Q6GTH7; Q86V36; Q9UH04

UniProt Related Accession #

Q12772

NCBI Official Full Name

sterol regulatory element binding protein-2

NCBI Official Synonym Full Names

sterol regulatory element binding transcription factor 2

NCBI Official Symbol

SREBF2

NCBI Official Synonym Symbols

SREBP2; bHLHd2; SREBP-2

UniProt Protein Name

Sterol regulatory element-binding protein 2

UniProt Synonym Protein Names

Class D basic helix-loop-helix protein 2; bHLHd2; Sterol regulatory element-binding transcription factor 2

UniProt Gene Name

SREBF2

UniProt Synonym Gene Names

BHLHD2; SREBP2; SREBP-2; bHLHd2

UniProt Entry Name

SRBP2_HUMAN

NCBI Protein Information

sterol regulatory element-binding protein 2; class D basic helix-loop-helix protein 2; sterol regulatory element-binding transcription factor 2

NCBI Summary for SREBP

This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

UniProt Comments for SREBP

SREBP-2: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway. Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. Belongs to the SREBP family.

Protein type: DNA-binding; Membrane protein, multi-pass; Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; C-8 sterol isomerase activity

Biological Process: cholesterol metabolic process; transcription, DNA-dependent; response to low density lipoprotein stimulus; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter

Research Articles on SREBP

1. Rating SREBF2 polymorphism did not reveal a relationship to the occurrence of ischemic heart disease in patients with obstructive sleep apnea, both in the whole group as well as separate subgroups.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SREBP elisa kit

Reference Product	PubMed Publications
SCAP elisa kit	>44 publications with SREBP and SCAP
INSIG1 elisa kit	>15 publications with SREBP and INSIG1
SP1 elisa kit	>12 publications with SREBP and SP1
INSIG2 elisa kit	>11 publications with SREBP and INSIG2
FDPS elisa kit	>2 publications with SREBP and FDPS
CREBBP elisa kit	>2 publications with SREBP and CREBBP

Pathways associated with SREBP elisa kit

Products by Pathway	Pathway Diagram
Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies	Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
Cholesterol Biosynthesis Pathway antibodies	Cholesterol Biosynthesis Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies	Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
PPARA Activates Gene Expression Pathway antibodies	PPARA Activates Gene Expression Pathway Diagram
Regulation Of Cholesterol Biosynthesis By SREBP (SREBF) Pathway antibodies	Regulation Of Cholesterol Biosynthesis By SREBP (SREBF) Pathway Diagram
Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies	Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
SREBF And MiR33 In Cholesterol And Lipid Homeostasis Pathway antibodies	SREBF And MiR33 In Cholesterol And Lipid Homeostasis Pathway Diagram

Diseases associated with SREBP elisa kit

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>84 publications with SREBP and Fatty Liver
Dyslipidemias Antibodies	>47 publications with SREBP and Dyslipidemias
Atherosclerosis Antibodies	>43 publications with SREBP and Atherosclerosis
Cardiovascular Diseases Antibodies	>38 publications with SREBP and Cardiovascular Diseases
Hyperlipidemias Antibodies	>37 publications with SREBP and Hyperlipidemias
Neoplasms Antibodies	>36 publications with SREBP and Neoplasms
Hypercholesterolemia Antibodies	>32 publications with SREBP and Hypercholesterolemia
Disease Models, Animal Antibodies	>29 publications with SREBP and Disease Models, Animal
Insulin Resistance Antibodies	>26 publications with SREBP and Insulin Resistance
Inflammation Antibodies	>23 publications with SREBP and Inflammation

Organs/Tissues associated with SREBP elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>179 publications with SREBP and Liver
Blood Antibodies	>85 publications with SREBP and Blood
Adipose Tissue Antibodies	>28 publications with SREBP and Adipose Tissue
Brain Antibodies	>23 publications with SREBP and Brain
Kidney Antibodies	>21 publications with SREBP and Kidney
Muscle Antibodies	>19 publications with SREBP and Muscle
Heart Antibodies	>19 publications with SREBP and Heart
Intestine Antibodies	>18 publications with SREBP and Intestine
Prostate Antibodies	>17 publications with SREBP and Prostate
Connective Tissue Antibodies	>15 publications with SREBP and Connective Tissue