Nitrotyrosine Antibody – Goat

Goat

0.5 ml in Freeze-dried powder.

0.8 mg / ml (lot specific)

Specifically binds to nitrated proteins. Dilution for immunoblot and ELISA range: 200 to 8,000.

Freeze-dried product should be stored refrigerated until opened. After opening, restore to 0.5 ml with distilled water. If it is not completely clear after standing for 1-2 Hours at room temperature, centrifuge the product. It is stable for several weeks at 4 degree C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20 degree C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol

Polyclonal antibody purified by nitrated protein-Sepharose affinity column, and conjugated with HRP.

In 50 mM PBS, 0.1M NaCl, pH 7.4 and 0.01% Thimersol.

10 mg / ml Bovine Serum Albumin.