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ECS Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3900623

The ECS epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized ECS epitope represents the enterokinase cleavage site DDDDK. More details

$400 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ECS (DYKDDDDK) Tag, Polyclonal Antibody
Full Product Name
Goat anti-ECS (DYKDDDDK) Tag Antibody Affinity Purified
Product Gene Name
anti-ECS antibody
Clonality
Polyclonal
Host
Goat
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Conjugation
Unconjugated

Product Categories/Family for anti-ECS antibody

Primary Antibodies
Applications Tested/Suitable for anti-ECS antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ECS antibody
WB: 1:1000-1:30,000
IP: 1-4ug/mg lysate
ICC: 1:100-1:400
ELISA: 1:1000-1:30,000; for coating plates 1:100-1:500

Immunocytochemistry (ICC) of anti-ECS antibody

Detection of DYKDDDDK-tagged Beta-Catenin by immunofluorescence. Samples: Human microvascular endothelial cells expressing DYKDDDDK-tagged beta-catenin following transient transfection. Antibody: Affinity purified goat anti-ECS (DYKDDDDK) (Cat. No. MBS3900623) used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).
Goat Anti-ECS Polyclonal Antibody – Cat# MBS3900623

Western Blot (WB) of anti-ECS antibody

Detection of ECS (DYKDDDDK)-tagged protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified goat anti-ECS (DYKDDDDK) antibody MBS3900623 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Goat Anti-ECS Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900623

Other Notes

Small volumes of anti-ECS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ECS purchase

MBS3900623 is an antibody targeting ECS. Also known as Goat anti-ECS (DYKDDDDK) Tag Antibody Affinity Purified. Host: Goat.
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