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EPLIN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3900853

Epithelial protein lost in neoplasm (EPLIN) is a cytoskeleton-associated protein found to be down-regulated in transformed prostate and breast cells. EPLIN is involved in actin dynamics and plays a role in the formation of stable actin filament structures. More details

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Product Overview

Online Datasheet in PDF
Product Name
EPLIN, Polyclonal Antibody
Full Product Name
Rabbit anti-EPLIN Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
LIMA1
Product Synonym Names
LIM domain and actin-binding protein 1; 1110021C24Rik; sterol regulatory element binding protein 3; SREBP3; LDLCQ8; EPLIN; Epithelial protein lost in neoplasm; epithelial protein lost in neoplasm beta; LIM domain and actin-binding protein 1; LIM domain and actin binding 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
67,117 Da
OMIM
608364
3D Structure
ModBase 3D Structure for Q9UHB6
Immunogen
Between 500 and 550
Conjugation
Unconjugated

Product Categories/Family for anti-EPLIN antibody

Applications Tested/Suitable for anti-EPLIN antibody
Immunoprecipitation (IP)
Application Notes for anti-EPLIN antibody
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-EPLIN antibody

Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody MBS3900853 (lot MBS3900853-2) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by a previous lot of this antibody (lot MBS3900853-1) and rabbit anti-EPLIN antibody MBS3900762 For blotting immunoprecipitated EPLIN, MBS3900762 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-EPLIN Polyclonal Antibody – Cat# MBS3900853

Other Notes

Small volumes of anti-EPLIN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPLIN purchase

MBS3900853 is an antibody targeting EPLIN. Also known as Rabbit anti-EPLIN Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EPLIN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
LIM domain and actin-binding protein 1 isoform 2
NCBI Official Synonym Full Names
LIM domain and actin binding 1
NCBI Official Symbol
LIMA1
NCBI Official Synonym Symbols
EPLIN; SREBP3
UniProt Protein Name
LIM domain and actin-binding protein 1
UniProt Synonym Protein Names
Epithelial protein lost in neoplasm
UniProt Gene Name
LIMA1
UniProt Synonym Gene Names
EPLIN; SREBP3
UniProt Entry Name
LIMA1_HUMAN
NCBI Protein Information
LIM domain and actin-binding protein 1; 1110021C24Rik; epithelial protein lost in neoplasm beta; sterol regulatory element binding protein 3

UniProt Comments for EPLIN

eplin: Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: focal adhesion; cytoplasm; plasma membrane; stress fiber; cleavage furrow; actin cytoskeleton

Molecular Function: actin monomer binding; actin filament binding; protein binding; zinc ion binding

Biological Process: actin filament bundle formation; ruffle organization and biogenesis; negative regulation of actin filament depolymerization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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