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RIF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901128

RAP1 interacting factor (RIF1) localizes to dysfunctional telomeres and functions in the S-phase checkpoint. In response to double-stranded breaks (DSBs), RIF1 functions in the ATM and ATR mediated response to DNA damage. RIF1 foci formation in response to… More details

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Product Overview

Online Datasheet in PDF
Product Name
RIF1, Polyclonal Antibody
Full Product Name
Rabbit anti-RIF1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
telomere-associated protein RIF1; RAP1 interacting factor homolog; rap1-interacting factor 1 homolog; Telomere-associated protein RIF1; replication timing regulatory factor 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
271,689 Da
OMIM
608952
3D Structure
ModBase 3D Structure for Q5UIP0
Immunogen
Between 1425 and 1475
Conjugation
Unconjugated

Product Categories/Family for anti-RIF1 antibody

Applications Tested/Suitable for anti-RIF1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-RIF1 antibody
WB: 1:10,000-1:25,000 (3-8% Tris-acetate gel recommended)
IP: 6ug/mg lysate
IHC: 1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-RIF1 antibody

Detection of human RIF1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RIF1 (Cat. No. MBS3901128 Lot5) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-RIF1 Polyclonal Antibody – Cat# MBS3901128

Immunoprecipitation (IP) of anti-RIF1 antibody

Detection of human Rif1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rif1 antibody (MBS3901128 lot 5) used for IP at 6 ug per reaction. Rif1 was also immunoprecipitated by a previous lot of this antibody (MBS3901128 lot 4) and a second antibody against a different epitope of Rif1 (MBS3901129). For blotting immunoprecipitated Rif1, MBS3901128 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Rabbit Anti-RIF1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3901128

Western Blot (WB) of anti-RIF1 antibody

Detection of human Rif1 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, K-562, HEK293T, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rif1 antibody (MBS3901128 lot 5) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-RIF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901128

Other Notes

Small volumes of anti-RIF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RIF1 purchase

MBS3901128 is an antibody targeting RIF1. Also known as Rabbit anti-RIF1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RIF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
telomere-associated protein RIF1 isoform 1
NCBI Official Synonym Full Names
replication timing regulatory factor 1
NCBI Official Symbol
RIF1
UniProt Protein Name
Telomere-associated protein RIF1
UniProt Synonym Protein Names
Rap1-interacting factor 1 homolog
UniProt Gene Name
RIF1
UniProt Entry Name
RIF1_HUMAN
NCBI Protein Information
telomere-associated protein RIF1; RAP1 interacting factor homolog; rap1-interacting factor 1 homolog

NCBI Summary for RIF1

This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

UniProt Comments for RIF1

RIF1: Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S- phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: chromosome, telomeric region; male pronucleus; cytoplasm; plasma membrane; female pronucleus; spindle; nucleus

Biological Process: stem cell maintenance; cell cycle; response to DNA damage stimulus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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