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PITX1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901136

PITX1 is a homeobox-containing transcription factor involved in the development of many organs. PITX1 has been demonstrated to play a critical role in pituitary organogenesis and can stimulate transcription from most pituitary hormone promoters. PITX1 has… More details

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Product Overview

Online Datasheet in PDF
Product Name
PITX1, Polyclonal Antibody
Full Product Name
Rabbit anti-PITX1 Antibody, Affinity Purified
Product Gene Name
anti-PITX1 antibody
Product Synonym Names
pituitary homeo box 1; POTX; pituitary otx-related factor; hindlimb expressed homeobox protein backfoot; pituitary homeobox 1; PTX1; LBNBG; Hindlimb-expressed homeobox protein backfoot; CCF; BFT; homeobox protein PITX1; paired-like homeodomain transcription factor 1; Pituitary homeobox 1; paired like homeodomain 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
119800
3D Structure
ModBase 3D Structure for P78337
Immunogen
Between 50 and 100
Conjugation
Unconjugated

Product Categories/Family for anti-PITX1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-PITX1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-PITX1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-PITX1 antibody

Detection of human PITX by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PITX antibody MBS3901136 (lot MBS3901136-2) used for IP at 6 ug per reaction. PITX was also immunoprecipitated by a previous lot of this antibody MBS3901136-1. For blotting immunoprecipitated PITX, MBS3901136 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-PITX1 Polyclonal Antibody – Cat# MBS3901136

Western Blot (WB) of anti-PITX1 antibody

Detection of human PITX by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PITX antibody MBS3901136 (lot MBS3901136-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-PITX1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901136

Other Notes

Small volumes of anti-PITX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PITX1 purchase

MBS3901136 is an antibody targeting PITX1. Also known as Rabbit anti-PITX1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PITX1. It may not necessarily be applicable to this product.

NCBI GeneID
5307
NCBI Accession #
NP_002644.3
UniProt Primary Accession #
P78337
UniProt Protein Name
Pituitary homeobox 1
UniProt Synonym Protein Names
Hindlimb-expressed homeobox protein backfoot; Homeobox protein PITX1; Paired-like homeodomain transcription factor 1
Protein Family
Pituitary homeobox
UniProt Gene Name
PITX1
UniProt Synonym Gene Names
BFT; PTX1
UniProt Entry Name
PITX1_HUMAN

UniProt Comments for PITX1

PITX1: May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Defects in PITX1 are a cause of congenital clubfoot (CCF); also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait. Belongs to the paired homeobox family. Bicoid subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: transcription factor complex; cytoplasm; nucleolus; nucleus

Biological Process: myoblast cell fate commitment; anatomical structure morphogenesis; transcription, DNA-dependent; pituitary gland development; cartilage development; branchiomeric skeletal muscle development; positive regulation of transcription from RNA polymerase II promoter; skeletal development; embryonic hindlimb morphogenesis

Disease: Liebenberg Syndrome; Clubfoot, Congenital, With Or Without Deficiency Of Long Bones And/or Mirror-image Polydactyly
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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