Rabbit Anti-RNF40 Polyclonal Antibody – Cat# MBS3901250

Detection of human RNF40 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNF40 antibody BL3315 (Cat. No. MBS3901250) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). RNF40 was also immunoprecipitated using rabbit anti-RNF40 antibodies BL3314 (Cat. No. MBS3901249) and BL3316 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

RNF40 Antibody – Rabbit Polyclonal

Name: RNF40 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3901250
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901250

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RNF40, Polyclonal Antibody

Full Product Name

Rabbit anti-RNF40 Antibody, Affinity Purified

Product Gene Name

anti-RNF40 antibody

Product Synonym Names

E3 ubiquitin-protein ligase BRE1B; RING-type E3 ubiquitin transferase BRE1B; ring finger protein 40, E3 ubiquitin protein ligase; RING finger protein 40; STARING; Rb-associated protein; BRE1B; BRE1 E3 ubiquitin ligase homolog B; 95 kDa retinoblastoma-associated protein; 95 kDa retinoblastoma protein binding protein; BRE1-B; RBP95; E3 ubiquitin-protein ligase BRE1B; ring finger protein 40

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

200ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

OMIM

607700

3D Structure

ModBase 3D Structure for O75150

Immunogen

Between 575 and 625

Conjugation

Unconjugated

Product Categories/Family for anti-RNF40 antibody

Primary Antibodies

Applications Tested/Suitable for anti-RNF40 antibody

Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-RNF40 antibody

WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Other Notes

Small volumes of anti-RNF40 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNF40 purchase

MBS3901250 is an antibody targeting RNF40. Also known as Rabbit anti-RNF40 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RNF40. It may not necessarily be applicable to this product.

NCBI GeneID

9810

NCBI Accession #

NP_055586.1

UniProt Primary Accession #

O75150

UniProt Protein Name

E3 ubiquitin-protein ligase BRE1B

UniProt Synonym Protein Names

95 kDa retinoblastoma-associated protein; RBP95; RING finger protein 40

Protein Family

E3 ubiquitin-protein ligase

UniProt Gene Name

RNF40

UniProt Synonym Gene Names

BRE1B; KIAA0661; BRE1-B; RBP95

UniProt Entry Name

BRE1B_HUMAN

UniProt Comments for RNF40

RNF40: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with RB1 and WAC. Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. Belongs to the BRE1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 16p11.2-p11.1

Cellular Component: nucleoplasm; neuron projection; membrane; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; protein homodimerization activity; mRNA 3'-UTR binding; zinc ion binding; ubiquitin protein ligase binding; protein complex binding; syntaxin-1 binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: histone monoubiquitination; ubiquitin-dependent protein catabolic process; regulation of mitotic cell cycle; histone H2B ubiquitination

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.