• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

rpL7a/SURF3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901272

rpL7a /SURF3 is a component of the 60S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RpL7a is a member of the L7AE family of ribosomal proteins that has been identified as… More details

$250 / 0.002 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
rpL7a/SURF3, Polyclonal Antibody
Full Product Name
Rabbit anti-rpL7a/SURF3 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
RPL7A
Product Synonym Names
PLA-X polypeptide; thyroid hormone receptor uncoupling protein; surfeit locus protein 3; TRUP; large ribosomal subunit protein eL8; L7A; 60S ribosomal protein L7a; surfeit 3; SURF3; 60S ribosomal protein L7a; ribosomal protein L7a
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
29,996 Da
OMIM
185640
3D Structure
ModBase 3D Structure for P62424
Predicted Reactivity
Rat, Chicken, Bovine
Immunogen
Between 216 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-rpL7a/SURF3 antibody

Applications Tested/Suitable for anti-rpL7a/SURF3 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-rpL7a/SURF3 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:100-1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-rpL7a/SURF3 antibody

Detection of human and mouse rpL7a (SURF3) by immunohistochemistry. Sample: FFPE sections of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-rpL7a (SURF3) (Cat. No. MBS3901272 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Rabbit Anti-rpL7a/SURF3 Polyclonal Antibody – Cat# MBS3901272

Western Blot (WB) of anti-rpL7a/SURF3 antibody

Detection of human rpL7a/SURF3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-rpL7a/SURF3 antibody MBS3901272 (lot MBS3901272-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-rpL7a/SURF3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901272

Western Blot (WB) of anti-rpL7a/SURF3 antibody

Detection of human rpL7a (SURF3) by western blot and immunoprecipitation/Mass Spectrometry. Samples: A) Whole cell lysate from HeLa (20 and 4 ug), MCF-7 (7; 20ug) and HEK293T (T; 20ug) cells. B) Cytosolic and nuclear extract (10 mg/IP) from HeLa S3 cells. Antibodies: Affinity purified rabbit anti-rpL7a (SURF3) antibody (Cat. No. MBS3901272) used for WB at 0.1 ug/ml (A) and for IP at 0.7 ug/mg lysate (B). Detection: Chemiluminescence with an exposure time of 5 sec (A) and vMALDI-LTQ and LC-MS/MS of Coomassie stained bands (B).
Rabbit Anti-rpL7a/SURF3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901272

Other Notes

Small volumes of anti-rpL7a/SURF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rpL7a/SURF3 purchase

MBS3901272 is an antibody targeting rpL7a/SURF3. Also known as Rabbit anti-rpL7a/SURF3 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for rpL7a/SURF3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
60S ribosomal protein L7a
NCBI Official Synonym Full Names
ribosomal protein L7a
NCBI Official Symbol
RPL7A
NCBI Official Synonym Symbols
L7A; TRUP; SURF3
UniProt Protein Name
60S ribosomal protein L7a
UniProt Synonym Protein Names
PLA-X polypeptide; Surfeit locus protein 3
UniProt Gene Name
RPL7A
UniProt Synonym Gene Names
SURF-3; SURF3
UniProt Entry Name
RL7A_HUMAN
NCBI Protein Information
60S ribosomal protein L7a

UniProt Comments for rpL7a/SURF3

RPL7A: Belongs to the ribosomal protein L7Ae family.

Protein type: Nuclear receptor co-regulator; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleus; polysomal ribosome

Molecular Function: protein binding; RNA binding

Biological Process: maturation of LSU-rRNA; mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000