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HEC1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901286

HEC1 (highly expressed in cancer 1) is a component of the kinetochore-associated NDC80 complex that is essential for maintaining kinetochore integrity, proper chromosome segregation, and spindle-checkpoint signaling. HEC1 has been shown to be required for the… More details

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Product Overview

Online Datasheet in PDF
Product Name
HEC1, Polyclonal Antibody
Full Product Name
Rabbit anti-HEC1 Antibody, Affinity Purified
Product Gene Name
anti-HEC1 antibody
Product Synonym Gene Name
NDC80
Product Synonym Names
kinetochore protein NDC80 homolog; HEC; TID3; retinoblastoma-associated protein HEC; NDC80 kinetochore complex component homolog; NDC80 homolog, kinetochore complex component; KNTC2; kinetochore-associated protein 2; kinetochore protein Hec1; kinetochore associated 2; hsNDC80; HsHec1; highly expressed in cancer, rich in leucine heptad repeats; HEC1; highly expressed in cancer protein; Kinetochore protein NDC80 homolog; NDC80, kinetochore complex component
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
73,913 Da
Chromosome Location
Chromosome: 18; NC_000018.9 (2571510..2616634). Location: 18p11.32
OMIM
607272
3D Structure
ModBase 3D Structure for O14777
Immunogen
Between 592 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-HEC1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-HEC1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-HEC1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Western Blot (WB) of anti-HEC1 antibody

Detection of human and mouse HEC1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HEC1 antibody BL3443 (Cat. No. MBS3901286) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). HEC1 was also immunoprecipitated by rabbit anti-HEC1 antibodies BL3440 (Cat. No. MBS3901285) and BL3441. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Rabbit Anti-HEC1 Polyclonal Antibody – Cat# MBS3901286

Other Notes

Small volumes of anti-HEC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HEC1 purchase

MBS3901286 is an antibody targeting HEC1. Also known as Rabbit anti-HEC1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HEC1. It may not necessarily be applicable to this product.

NCBI GI #
5174457
NCBI GeneID
10403
NCBI Accession #
NP_006092.1
UniProt Primary Accession #
O14777
UniProt Secondary Accession #
Q6PJX2
UniProt Related Accession #
O14777
GenBank Nucleotide #
NM_006101.2
NCBI Official Full Name
kinetochore protein NDC80 homolog
NCBI Official Synonym Full Names
NDC80 kinetochore complex component
NCBI Official Symbol
NDC80
NCBI Official Synonym Symbols
HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
UniProt Protein Name
Kinetochore protein NDC80 homolog
UniProt Synonym Protein Names
Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC
Protein Family
Transcription factor
UniProt Gene Name
NDC80
UniProt Synonym Gene Names
HEC; HEC1; KNTC2; HsHec1
UniProt Entry Name
NDC80_HUMAN
NCBI Protein Information
kinetochore protein NDC80 homolog; kinetochore associated 2; kinetochore protein Hec1; kinetochore-associated protein 2; highly expressed in cancer protein; retinoblastoma-associated protein HEC; NDC80 kinetochore complex component homolog; NDC80 homolog

NCBI Summary for HEC1

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
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UniProt Comments for HEC1

HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: kinetochore; membrane; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: mitosis; mitotic spindle organization and biogenesis; establishment of mitotic spindle orientation; mitotic sister chromatid segregation; attachment of spindle microtubules to kinetochore during mitosis; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation
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Research Articles on HEC1

1. NDC80, NUF2 and PTN were significantly aberrantly overexpressed in serous adenocarcinomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HEC1 antibody

 Reference Product  PubMed Publications
 NUF2 antibody  >42 publications with HEC1 and NUF2
 MIS12 antibody  >35 publications with HEC1 and MIS12
 SPC25 antibody  >26 publications with HEC1 and SPC25
 ZWINT antibody  >8 publications with HEC1 and ZWINT
 MAD2L1 antibody  >7 publications with HEC1 and MAD2L1
 NEK2 antibody  >4 publications with HEC1 and NEK2
 MAD1L1 antibody  >4 publications with HEC1 and MAD1L1
 AURKA antibody  >1 publications with HEC1 and AURKA

Pathways associated with anti-HEC1 antibody

 Products by Pathway  Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 PLK1 Signaling Events Pathway antibodies  PLK1 Signaling Events Pathway Diagram
 Resolution Of Sister Chromatid Cohesion Pathway antibodies  Resolution Of Sister Chromatid Cohesion Pathway Diagram
 Separation Of Sister Chromatids Pathway antibodies  Separation Of Sister Chromatids Pathway Diagram

Diseases associated with anti-HEC1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >20 publications with HEC1 and Neoplasms
 Carcinoma Antibodies  >5 publications with HEC1 and Carcinoma
 Ovarian Neoplasms Antibodies  >4 publications with HEC1 and Ovarian Neoplasms
 Breast Neoplasms Antibodies  >4 publications with HEC1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >3 publications with HEC1 and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >3 publications with HEC1 and Lung Neoplasms
 Adenocarcinoma Antibodies  >3 publications with HEC1 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >2 publications with HEC1 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >1 publications with HEC1 and Nervous System Diseases
 Precancerous Conditions Antibodies  >1 publications with HEC1 and Precancerous Conditions

Organs/Tissues associated with anti-HEC1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3 publications with HEC1 and Blood
 Lung Antibodies  >3 publications with HEC1 and Lung
 Heart Antibodies  >2 publications with HEC1 and Heart
 Stomach Antibodies  >2 publications with HEC1 and Stomach
 Brain Antibodies  >2 publications with HEC1 and Brain
 Kidney Antibodies  >1 publications with HEC1 and Kidney
 Cervix Antibodies  >1 publications with HEC1 and Cervix
 Ovary Antibodies  >1 publications with HEC1 and Ovary
 Skin Antibodies  >1 publications with HEC1 and Skin
 Testis Antibodies  >1 publications with HEC1 and Testis
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