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MIS12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901290

MIS12 is an inner kinetochore protein found in a complex with Dsn1, Nnf1, and Nsl1 that functions in kinetochore assembly. This MIS12 complex has been demonstrated to be required for proper chromosome alignment and cell cycle progression. More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MIS12, Polyclonal Antibody
Full Product Name
Rabbit anti-MIS12 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
MIS12 homolog; MTW1; MIS12, MIND kinetochore complex component, homolog; homolog of yeast Mis12; hMis12; 2510025F08Rik; protein MIS12 homolog; KNTC2AP; Protein MIS12 homolog; MIS12, kinetochore complex component
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
24,140 Da
OMIM
609178
3D Structure
ModBase 3D Structure for Q9H081
Predicted Reactivity
Bovine, Orangutan
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-MIS12 antibody

Applications Tested/Suitable for anti-MIS12 antibody
Immunoprecipitation (IP)
Application Notes for anti-MIS12 antibody
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-MIS12 antibody

Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody MBS3901290 (lot MBS3901290-2) used for IP at 3 ug per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody MBS3901291. For blotting immunoprecipitated MIS12, MBS3901291 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-MIS12 Polyclonal Antibody – Cat# MBS3901290

Other Notes

Small volumes of anti-MIS12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIS12 purchase

MBS3901290 is an antibody targeting MIS12. Also known as Rabbit anti-MIS12 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MIS12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens MIS12 kinetochore complex component (MIS12), transcript variant 2, mRNA
NCBI Official Synonym Full Names
MIS12 kinetochore complex component
NCBI Official Symbol
MIS12
NCBI Official Synonym Symbols
MTW1; hMis12; KNTC2AP; 2510025F08Rik
UniProt Protein Name
Protein MIS12 homolog
Protein Family
UniProt Gene Name
MIS12
UniProt Entry Name
MIS12_HUMAN
NCBI Protein Information
protein MIS12 homolog; MIS12, MIND kinetochore complex component, homolog; homolog of yeast Mis12

UniProt Comments for MIS12

MIS12: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Belongs to the mis12 family.

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: nucleus; cytosol; MIS12/MIND type complex

Molecular Function: protein binding

Biological Process: mitosis; kinetochore assembly; cell division; mitotic cell cycle; chromosome segregation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-MIS12 antibody

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