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ZWINT-1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901295

Yeast 2-hybrid and localization studies show that human ZW10 interacting protein 1 (ZWINT-1) interacts with HZw10 in active centromeres and may play a role in directing HZw10 to the kinetochore during prometaphase. HZw10 is a component of the mitotic… More details

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Product Overview

Online Datasheet in PDF
Product Name
ZWINT-1, Polyclonal Antibody
Full Product Name
Rabbit anti-ZWINT-1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
ZWINT
Product Synonym Names
HZwint-1; ZW10 interactor, kinetochore protein; zwint-1; ZWINT1; ZW10-interacting protein 1; SNAP25 interacting protein of 30 kDa; human ZW10 interacting protein-1; KNTC2AP; SIP30; ZW10 interactor; ZW10 interactor; ZW10 interacting kinetochore protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
25,544 Da
OMIM
609177
3D Structure
ModBase 3D Structure for O95229
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-ZWINT-1 antibody

Applications Tested/Suitable for anti-ZWINT-1 antibody
Immunoprecipitation (IP)
Application Notes for anti-ZWINT-1 antibody
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-ZWINT-1 antibody

Detection of human ZWINT-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZWINT-1 antibody BL3450 (Cat. No. MBS3901295) used for IP at 3 ug/mg lysate. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody BL3453 (Cat. No. MBS3901296). For blotting immunoprecipitated ZWINT-1, BL3453 was used at 1 ug/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-ZWINT-1 Polyclonal Antibody – Cat# MBS3901295

Other Notes

Small volumes of anti-ZWINT-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZWINT-1 purchase

MBS3901295 is an antibody targeting ZWINT-1. Also known as Rabbit anti-ZWINT-1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ZWINT-1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ZW10 interactor isoform a
NCBI Official Synonym Full Names
ZW10 interacting kinetochore protein
NCBI Official Symbol
ZWINT
NCBI Official Synonym Symbols
ZWINT1; KNTC2AP; HZwint-1
UniProt Protein Name
ZW10 interactor
UniProt Synonym Protein Names
ZW10-interacting protein 1; Zwint-1
Protein Family
UniProt Gene Name
ZWINT
UniProt Synonym Gene Names
Zwint-1
UniProt Entry Name
ZWINT_HUMAN
NCBI Protein Information
ZW10 interactor; zwint-1; human ZW10 interacting protein-1; ZW10 interactor, kinetochore protein

NCBI Summary for ZWINT-1

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for ZWINT-1

ZWINT: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q21-q22

Cellular Component: cytoplasm; cytosol; kinetochore

Molecular Function: protein binding; protein N-terminus binding

Biological Process: establishment of cellular localization; mitotic cell cycle checkpoint; mitotic sister chromatid segregation; sister chromatid cohesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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